Normalizing Seahorse™ XF Data with Label-Free Cell Counting
One of the critical factors to ensure accurate Seahorse™ analysis results are uniform cell seeding density. What happens if seeding is not uniform? Currently, researchers normalize Seahorse™ results using protein analysis assay such as BCA to minimize the effects of non-uniform cell seeding. Protein analysis is a widely used method due to its availability in biological laboratories, however,