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Plasmid MAXI prep for inVitro translation
Plasmid MAXI prep Grow E. coli in 100 ml LB + antibiotic (e.g. 100ug/ml ampicillin final). Spin 5000 RPM or more for 10min and just keep pellet. Add 5 ml of solution I ( glucose 0.450 g , 1.25 ml …