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T4 DNA ligase recipe
2X Rapid Ligation Buffer: 132 mM Tris-HCl, 20 mM MgCl2, 2 mM DTT, 2 mM ATP, 15% PEG 6000, pH 7.6 For ligation of long DNA fragments, prepare the buffer below: 10X T4 DNA Ligase Buffer: 500 …