Supplemented Cell Culture Media: An Effective Approach for Enhanced Monoclonal Antibody Production | PSM | Nonprofit Scientific Publishing
EDITORIAL Monoclonal antibodies (mAbs) are broadly used in medical research, therapeutics and diagnostics (Jorgensen et al., 2007). mAbs are among the best-selling class of biopharmaceutics (Ho et al., 2013). The in vitro hybridoma culture is an appropriate method for monoclonal antibody production using the culture supernatant (Falkenberg et al., 1995; Sen and Roychoudhury, 2013). The production of recombinant therapeutic biologics in cell culture is a rapidly growing industry, creating many opportunities for bioprocess engineering research and development. To meet this increasing demand for therapeutic biologics, the primary goal of increasing the yield of bioprocesses, either by increasing maximum cell densities of cultures, or improving hybridoma cell culture that requires optimizing the nutrient medium in order to support cell growth, reduce cell death, and enhance mAb production (Kelley, 2009). Supplemental feeding of amino acids has proven effective for improving cell viability