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Molecular Characterization of Xanthomonas oryzae pv. oryzae Isolates and its Resistance Sources in Rice Germplasm | PSM | Nonprofit Scientific Publishing
Abstract: Bacterial leaf blight (BLB) of rice, the most notorious and horrendous disease of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo) andcontinue to evolve covering more area and rice cultivars. Keeping in view the escalating virulence in pathogen population, current study was conducted to determine the genetic diversity in pathogen population and to search new sources of resistance in rice germplasm. The samples collected during the survey from different rice growing areas of Punjab were provided by the Rice Pathology group of NIAB. Then these samples were plated to isolate and purify the pathogen causing bacterial blight disease Xanthomonas oryzae pv. oryzae. The genetic diversity of the pathogens from different areas of the Punjab was determined through the repetitive DNA element with primers JEL1 and JEL2. During this study, twenty seven (27) varieties/lines were screened for resistance under artificial inoculation conditions against BLB. Plants were artificially