Alice: Madness Returns - It Was No Accident:

“This is one of the more shocking reveals in the game. A friend of mine warned me about this reveal, and when he told me that the doctor raped Alice’s sister, I was speechless. This is the type of things that usually get me off guard in videogames, because I don’t tend to expect something so awful to be even mentioned. The level itself is quite short, so there isn’t much to say about it. It has a big focus on puzzles and platforming.”

PS: the gameplay used for this gifset is not mine. The original video comes from the user: J, on Youtube.