western blot

Honest science clickbait

23 questions we don’t have answers to

12 mind blowing pictures of protein gels

you HAVE to see how sexy these 7 western blots look

21 papers you should have read before you started your project

17 pieces of data that just don’t add up

9 unpublishable results

you’ll NEVER believe reviewer 3′s response to this paper submission

science FAIL: 11 times your experiment just didn’t work

Relief to those who perform tedious western blots!!!!

“Biochemists may soon have more time on their hands. Researchers in Michigan have speeded up Western blotting, a biochemistry workhorse for protein separations, by coupling it to an analytical chemistry mainstay, capillary electrophoresis (CE) (Anal. Chem., DOI 10.1021/ac102671n). The new technique produces data in a quarter of the time, and with a fraction of the sample, that Western blots normally use.”

“The result is eliminating the labor-intensive gel-to-membrane transfer.”


Blot, blot, western baby. Figure one will be amazing.

I stood pipetting for a good 3 hours straight in preparation for the largest series of western blots I’ve ever ran requiring a total of 108 wells split among six gels for the lack of a giant contraption to run such a gel, if I could even make something like that.

You’d think that my arms and shoulders would be sore as heck from all of the repetitive motion, especially since I was working at blazing speed to get all of my samples into loading lysates before I had to leave, but amazingly, they’re not. I think I am the perfect height for working on the lab benches while standing up. Who needs a class on ergonomics, when standing works out perfectly for me. 

But my, do I have my work cut out for me next week.