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Dyeing The Dead - EP 7: OOPS!! I Made A Mistake!

Hey everyone! Welcome back to another episode of #DyeingTheDead.

This video is about the problem(s) I’ve encountered along my journey of Diaphonization. A few episode ago, during the Rehydration step, I had to go out of town for a few weeks. Well, since I wasn’t going to be working on my specimens I thought “Hey, Why don’t I just leave them in the distilled water until I get back?” That, my friends, was the WRONG DECISION.

**I would like to reiterate to EVERYONE WHO WATCHES MY VIDEOS that this is my FIRST TIME GOING THROUGH THE PROCESS OF DIAPHONIZATION. I have NEVER done this before, EVER in my life. I ask that you all PLEASE, DON’T MISTAKE THIS SERIES AS A 100% rock solid TUTORIAL that’s going to produce the amazing, professional specimens like the ones you can search on online.**

As I am doing this for the first time so I don’t know what kind of specimens I’m going to produce win the end I don’t really know what to even expect at each step/stage. I can only tell you all the results that I’m getting after following a protocol. I have not worked out all the problem/kinks yet.

I am filming my journey through this process to show you all (who are watching my series and videos) HOW I am doing this process, HOW I’m preparing the solutions, HOW many days I leave my specimens in said solution(s), and the results I am getting.

Here’s What Happened:
SO, before I moved on the Trypsin Digestion step, I left my specimens in the distilled water for almost two weeks before transferring them into the trypsin solution. When I put my specimens into the Trypsin solution after a day (or two or three) I noticed that they were still “brown” by the spine and rib cage. The muscles were also not very transparent, when they should have been after THREE days in the trypsin solution. My specimens did not clear as well and didn’t look as transparent as they should have. This is attributed to the “Formalin Fixation” being a REVERSIBLE PROCESS. The formalin fixation reversed itself and the mice actually started decomposing in the distilled water. (They didn’t have a strong odor or anything). Leaving the specimens in the distilled water for such a long period of time is where I went wrong.. I also did not immediately proceed to the Trypsin Digestion Step.

This is definitely disappointing and a little upsetting because I had been working on these specimens (and I’m still working on them). It definitely sucks a little bit. I just can’t believe that I left my specimens in distilled water and didn’t think about putting them into something that would keep them from decomposing.

NOT TO WORRY THOUGH!!!
I have a THIRD MOUSE that I have been working on!!! I am still going to be using my original two specimens that I started with! (: They will go through this entire process until the very end! The third mouse that I have been working on is caught up with the other two and will continue the process until the end! This mouse went directly from the Rehydration step and into the Trypsin Digestion; there were no lag days in between. I have pretty high hopes for this mouse as it cleared VERY WELL in the trypsin solution which I am very happy about! Overall, I’m just really hoping that all three of my specimens turn out. That would be awesome!

COMPARING THE MICE:
In this video, I also show a side-by-side comparison of two mice. The mouse on the left was left in distilled water for a few weeks, and the other (mouse on the right) had gone directly into the Trypsin Solution. There is a significantly HUGE difference in their appearances. The mouse (on the right) that went directly to trypsin step cleared significantly easier. The mouse was A LOT more transparent (muscles and tissues), the spine was clearly visible, and you could see the Alcian Blue Cartilage Stain.

**In my opinion: I feel that this mouse is what your specimens should look like!!! I’m part of a forum on Taxidermy.net and they were able to let me know that the mouse on the right is how your mice are supposed to look after being in the tryspin.

So I would say that when going through all the steps DON’T HAVE ANY LAG DAYS IN BETWEEN STEPS! You want to go directly from one step to the next.

By: Taco Kel.
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Dyeing The Dead - EP 6: Trypsin Digestion

In this video I will show you how to prepare the Trypsin Digestion Solution! This is where I’m going to be digesting and softening the muscles and tissues of my specimens.

Before I moved on to this step I left my specimens in the distilled water for almost two weeks before transferring them into the trypsin solution. So, when looking at the specimens after a day (or two or three) in the trypsin I noticed that they were still “brown” by the spine and rib cage. The muscles were also not very transparent. This is attributed to the fact that I did not immediately proceed to this step. DON’T MAKE THIS MISTAKE!!!!!

Once you complete the Rehydration steps you need to IMMEDIATELY go onto this step! There should be no lagging time between any of the steps. You will produce better specimens!

By: TacoKel.
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