sulphite

themathomhouse  asked:

So you probably don't remember but with my Crohn's you told me to avoid sulphites? Studies have now shown a link between that and digestive problems

Ooooh my god fucking finally. I need to find the new studies and print them out so I can hit my GI doctor on the head for laughing at me.

It’s also said that dwarfs have two hundred words for rock.
  
They don’t. They have no words for rock, in the same way that fish have no words for water. They do have words for igneous rock, sedimentary rock, metamorphic rock, rock underfoot, rock dropping on your helmet from above, and rock which looked interesting and which they could have sworn they left here yesterday. But what they don’t have is a word meaning “rock.” Show a dwarf a rock and he sees, for example, an inferior piece of crystalline sulphite of barytes.
—  Witches Abroad by Terry Pratchett

Legit food for Transformers:

  • Beryllium baloney
  • Cesium salami
  • Chase chips
  • Chocolate covered oil drums (Galvatron has a weakness for these)
  • Chocolate covered wheel drums
  • Chrome-alloy Cake
  • Chrome-alloy Pie
  • Chromia’s Pizza
  • Cyber-grapefruit
  • Cyber-tomatoes
  • Energon chankonabe (stew)
  • Energon goodies
  • Eneron goodie cake
  • Garbage O’s (Wreck-Gar brand cereal)
  • Lead sulphite crystals
  • Mendu (soup)
  • Oil cake
  • Plutonium pepperoni
  • Servo Salad (Warning: made from real servos. Blitzwing has his with iron filings)
  • Space Carrots
  • Zygotic fermented slime slurpee 

Ingredients

  • Grated Gears
  • Iron filings
  • mercury sauce

Clostridium perfringens

So the bacteria that I am currently working with is called C.perfringens and is a common soil bacteria that is also found in association with mammals as a commensal and sometimes as a pathogen. There are five serotypes (A-E), each associated with a set of different diseases and target organism which is either a mammal or poultry. C.perfringens is capable of producing up to 16 different toxins, although these are rarely expressed all at once. It is a saprophyte, so decays dead matter - hence most of its toxins are hydrolytic enzymes. The most common diseases caused by C.perfringens is food poisoning and necrotic enteritis (chickens). 

It is also the fastest growing bacteria, with a doubling time of 10 minutes (E.coli’s doubling time is 20 minutes). It is an obligate anaerobe which makes it tricky to grow. I have to essentially streak it out onto TSC agar and then add a second layer of agar on top of that to create a highly anaerobic microenvironment. I also need to leave these microbes in an anaerobic chamber over night to completely remove any possible source of oxygen from their surroundings. 

The final thing I want to mention is the TSC media I use to grow them. This media contains a source of sulphite which C.perfringens is able to reduce which is what causes the bacteria to show up black on these plates. They are one of the few Clostridia that can reduce sulphite, so this media acts as a differential media for C.perfringens. 

Sam