serotype

New Salmonella Serotype

Date:
January 16, 2015
Source:
Texas Tech University
Summary:
A new serotype of the salmonella bacteria has been discovered by Texan researchers. Because convention calls for a new serotype to be named after the city in which it is discovered, this one will be called Salmonella Lubbock. And while Lubbock is known for many things, like being the home of Buddy Holly, this new honor will provide new avenues for research into the bacteria’s prevention, researchers suggest.

http://www.sciencedaily.com/releases/2015/01/150116143835.htm?utm_source=feedburner&utm_medium=email&utm_campaign=Feed%3A+sciencedaily%2Fhealth_medicine%2Ffoodborne_illness+%28Foodborne+Illness+News+—+ScienceDaily%29

Specific gene expression in mouse cortical astrocytes is mediated by a 1740bp-GFAP promoter-driven combined adeno-associated virus2/5/7/8/9.

PubMed: Specific gene expression in mouse cortical astrocytes is mediated by a 1740bp-GFAP promoter-driven combined adeno-associated virus2/5/7/8/9.

Neurosci Lett. 2015 Mar 13;

Authors: Meng X, Yang F, Ouyang T, Liu B, Wu C, Jiang W

Abstract
We sought to demonstrate the in vivo transduction efficiency and tropism range in astrocytes of a combined-serotype adeno associated virus (AAV2/5/7/8/9). To control expression of enhanced green fluorescent protein (EGFP), a 1740bp glial fibrillary acidic protein (GFAP) promoter was obtained and ligated into vectors of each AAV serotype (2/5/7/8/9). Purified AAVs were then injected into the somatosensory cortex of C57BL/6J mice. Cell-type specific antibodies and subsequent immunofluorescence were used to identify astrocytes (GFAP), neurons (neuronal nuclear antigen, NeuN), microglia (ionized calcium-binding adapter molecule 1, Iba1), and oligodendrocytes (myelin basic protein, MBP), whereby, EGFP expression was measured in each cell type at 1-4 weeks post-injection. Our results indicated that the majority of astrocytes expressed EGFP, while only a small number of neurons expressed EGFP. Both microglia and oligodendrocytes lacked EGFP expression after viral injection. Quantitative analyses revealed that the percentage of EGFP-positive astrocytes was about 98% after viral injection, while the EGFP-positive neuronal percentage was less than 2%. Thus, this study shows that using a combined-serotype AAV carrying a 1740bp GFAP promoter results in successful, cell-type specific infection of the central nervous system, with robust gene expression in murine astrocytes.

PMID: 25778419 [PubMed - as supplied by publisher] http://dlvr.it/91R2vk