schrimpf

Methylation-based classification of benign and malignant peripheral nerve sheath tumors.

PubMed: Methylation-based classification of benign and malignant peripheral nerve sheath tumors.

Acta Neuropathol. 2016 Feb 8;

Authors: Röhrich M, Koelsche C, Schrimpf D, Capper D, Sahm F, Kratz A, Reuss J, Hovestadt V, Jones DT, Bewerunge-Hudler M, Becker A, Weis J, Mawrin C, Mittelbronn M, Perry A, Mautner VF, Mechtersheimer G, Hartmann C, Okuducu AF, Arp M, Seiz-Rosenhagen M, Hänggi D, Heim S, Paulus W, Schittenhelm J, Ahmadi R, Herold-Mende C, Unterberg A, Pfister SM, von Deimling A, Reuss DE

Abstract
The vast majority of peripheral nerve sheath tumors derive from the Schwann cell lineage and comprise diverse histological entities ranging from benign schwannomas and neurofibromas to high-grade malignant peripheral nerve sheath tumors (MPNST), each with several variants. There is increasing evidence for methylation profiling being able to delineate biologically relevant tumor groups even within the same cellular lineage. Therefore, we used DNA methylation arrays for methylome- and chromosomal profile-based characterization of 171 peripheral nerve sheath tumors. We analyzed 28 conventional high-grade MPNST, three malignant Triton tumors, six low-grade MPNST, four epithelioid MPNST, 33 neurofibromas (15 dermal, 8 intraneural, 10 plexiform), six atypical neurofibromas, 43 schwannomas (including 5 NF2 and 5 schwannomatosis associated cases), 11 cellular schwannomas, 10 melanotic schwannomas, 7 neurofibroma/schwannoma hybrid tumors, 10 nerve sheath myxomas and 10 ganglioneuromas. Schwannomas formed different epigenomic subgroups including a vestibular schwannoma subgroup. Cellular schwannomas were not distinct from conventional schwannomas. Nerve sheath myxomas and neurofibroma/schwannoma hybrid tumors were most similar to schwannomas. Dermal, intraneural and plexiform neurofibromas as well as ganglioneuromas all showed distinct methylation profiles. Atypical neurofibromas and low-grade MPNST were indistinguishable with a common methylation profile and frequent losses of CDKN2A. Epigenomic analysis finds two groups of conventional high-grade MPNST sharing a frequent loss of neurofibromin. The larger of the two groups shows an additional loss of trimethylation of histone H3 at lysine 27 (H3K27me3). The smaller one retains H3K27me3 and is found in spinal locations. Sporadic MPNST with retained neurofibromin expression did not form an epigenetic group and most cases could be reclassified as cellular schwannomas or soft tissue sarcomas. Widespread immunohistochemical loss of H3K27me3 was exclusively seen in MPNST of the main methylation cluster, which defines it as an additional useful marker for the differentiation of cellular schwannoma and MPNST.

PMID: 26857854 [PubMed - as supplied by publisher] http://dlvr.it/KT44nf

Russians and Syrians Achieve Breakthrough in Aleppo

Russians and Syrians Achieve Breakthrough in Aleppo

Originally Appeared at German Economic News. Translated from the German by Werner Schrimpf With its support of the Russian Air Force, Syria’s army has achieved a strategically important breakthrough near Aleppo.  That could mean that main supply routes from Turkey to the terrorists are now cut off. Syria’s army together with its allies have successfully fought against IS terrorist posts in the…

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German Businesses Keep Calling For End to Russia Sanctions

German Businesses Keep Calling For End to Russia Sanctions

Originally Appeared at Sputnik Deutschland. Translated from the Germany by Werner Schrimpf. Alfred Gaffal, President of the Bavarian Business Association, wrote in an article published Tuesday in the German newspaper “Bayernkurier,” that  the prolongation of EU-sanctions against Russia is a severe mistake which will hurt the EU. It will especially hurt the German Federal State Bavaria and Russia.…

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Angela 'Mom' Merkel as the Mother of All Sanctions

Angela ‘Mom’ Merkel as the Mother of All Sanctions

Originally Appeared at German-Russian Economic News. Translated from the German by Werner Schrimpf [Translator’s note: “Mom” is the disparaging nickname Ms. Merkel has earned in Germany] German Chancellor Merkel seems to be more and more the driving force behind the economic EU-sanctions against Russia that are extended every six months on a regular basis.  And she seems to be pleased to be…

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Austrian Vice Chancellor, Head of Raiffeisen Decry Sanctions in Moscow

Austrian Vice Chancellor, Head of Raiffeisen Decry Sanctions in Moscow

Originally Appeared at Sputnik Deutschland. Translated from the German from Werner Schrimpf During his visit with Moscow, Austria’s Federal Minister of the Economy and Vice Chancellor, Mr. Reinhold Mitterlehner, discussed flawed trade relations between both countries due to the EU sanctions against Russia. Prior to his visit, die vice chancellor explained that unless the economic relations…

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