This animal can reach a total length of at least 60 mm. It has a grey-green to dark green body with blackish longitudinal wrinkles running down the length of the body. A different color form with pale yellowish green background has been observed around Taiwan. The rhinophores and gills are typically black.
Nembrotha chamberlaini is white with streaks of black and occasionally yellow splashed across the upper mantle. It has very distinctive bright red gills and rhinophores. The foot and mouth parts are typically light-purple. This nudibranch has a very characteristic color pattern which is typical of species that display warning coloration to other species.
Nembrotha chamberlaini is easily confused with Nembrotha aurea and Nembrotha purpureolineata. All three species have a similar range of color variation although N. aurea often has orange patches not present in Nembrotha chamberlaini.
N. chamberlaini can reach a length of 100 mm.
This species feeds on ascidians and tunicates. It has been seen feeding on the bright-blue ascidian, Rhopalaea sp, as well as other ascidians Clavelina sp., & Oxycorynia sp.