nucleosomes

2

It has already been determined that silencing near tRNA genes requires nucleolar localisation through work with yeast, and now a follow up paper from David Engelke’s lab at the University of Michigan tracks the process ‒ tRNA gene mediated (tgm) silencing ‒ to the nucleosomes.

Elaborating on what makes this form of silencing distinct from other forms of transcriptional silencing or boundary element (cis-acting elements which also serve a structural role) function in yeast, the group discuss models for communication between the tRNA gene transcription complexes and local chromatin.

These RNA polymerase III transcription units are dispersed in high copy throughout nuclear genomes and act counter to RNA polymerase II transcription in their immediate vicinity on a chromosome. 

In some respects tgm silencing may be incompatible with other forms of silencing, since tRNA genes are able to block propagation of silenced chromatin states caused by other silencing mechanisms. It is not clear what the relationship is between tgm silencing and this “insulator” or “boundary element” function.

To test the possible involvement of nucleosomes in an unbiased manner, we screened a comprehensive library of alanine substitution mutations in the four core histones for the ability to release tgm silencing. Over 10% of the substitutions strongly release the silencing, with many residing in the histone H3 and H4 N-terminal tails. In addition, a large group of alleviating mutations defines a surface on the nucleosome disk that has not been implicated in other silencing forms. In light of these observations, we also tested additional mutations in a broad group of chromatin modification and remodeling enzymes, and find that the Rpd3 and Hos1 histone deacetylases, the Glc7 phosphatase, and the RSC nucleosome remodeling activity are also required for silencing.

As can be seen from the images above,

The identified residues map to three general areas of the nucleosome structure: the N-terminal tails of H3 and H4, other residues likely in near contact with the DNA near the nucleosome dyad, and a broad surface on the nucleosome disk face.

The H3 and H4 tails are particularly intensive regions of post-translational modifications that affect transcription and have the densest clusters of mutations that alleviate silencing. They emerge from the nucleosome core nearly opposite each other across the DNA helix and can interact with both the DNA and external proteins.

The left view is from the edge of the disc to optimise visualisation of residues in contact with DNA, while the view alongside it optimises visualisation of residues on the disc surface.

Light blue = DNA, Grey = histones (except residues affecting tgm silencing) : Blue = H2A, Cyan = H2B, Yellow = H3, Red = H4.

An “intriguing possibility” arises from the finding that Maf1, a direct target of TOR signaling which represses RNA pol. III, is required for tgm silencing.

When MAF1 is deleted, tRNA transcription increases, which we originally expected would intensify silencing near tRNA genes. When tgm silencing was instead abolished by maf1Δ , it indicated Maf1 was exerting an influence beyond its physical interaction with pol III and the tRNA gene-bound transcription factors. A link between Maf1 and RSC [chromatin remodelling complex] is thought to be mediated through the Rsc9 subunit, consistent with the involvement of the RSC complex in transcriptional regulation in response to TOR signaling.
Unlike boundary element function, which requires only bound TFIIIC, tgm silencing seems to require the presence of the full RNA polymerase III complex (pol III, TFIIIC, TFIIIB, 1), containing at least 25 polypeptides. Thus, it is not surprising that silencing near the tRNA genes involves interactions with activities not observed in boundary function.

Since both tRNA genes and some tRNA-derived short interspersed elements (SINEs) have been shown to have boundary element properties in larger eukaryotic genomes, it will be interesting to examine whether the other interactions with local chromatin structure are retained as well in these highly reiterated elements.

Good et al., “Silencing near tRNA genes is nucleosome-mediated and distinct from boundary element function”. Gene, vol 526, pp. 7‒15 (2013)

Nucleosome hopping and sliding kinetics determined from dynamics of single chromatin fibers in Xenopus egg extracts (2007)

Chromatin function in vivo is intimately connected with changes in its structure: a prime example is occlusion or exposure of regulatory sequences via repositioning of nucleosomes. Cell extracts used in concert with single-DNA micromanipulation can control and monitor these dynamics under in vivo-like conditions. We analyze a theory of the assembly–disassembly dynamics of chromatin fiber in such experiments, including effects of lateral nucleosome diffusion (“sliding”) and sequence positioning. Experimental data determine the force-dependent on- and off-rates as well as the nucleosome sliding diffusion rate. The resulting theory simply explains the very different nucleosome displacement…

  • Nucleosome displacement in transcription (2007)
    • “It is becoming increasingly clear that the eukaryotic transcriptional machinery is adapted to exploit the presence of nucleosomes in very sophisticated ways.”

…kinetics observed in constant-force and constant-pulling velocity experiments. We also show that few-piconewton tensions comparable to those generated by polymerases and helicases drastically affect nucleosome positions in a sequence-dependent manner and that there is a long-lived structural “memory” of force-driven nucleosome rearrangement events.

im so homo i have no heterochromatin and all my genes are transcribable however that also means that i have no histones and am unable to form nucleosomes which leaves me with free dna resulting in extreme fragility and a lack of transcription regulation
cells that lack histones have been shown to be susceptible to genetic damage and early death

Discovery of a new form of DNA modification in insects, worms and algae

Discovery of a new form of DNA modification in insects, worms and algae

Scientists at the University of Chicago, Harvard University and the Chinese Academy of Sciences have described the surprising discovery and function of a new DNA modification in insects, worms and algae.

Common DNA modifications occur through methylation, (more…)

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WHAT ARE NUCLEOSOMES AND HISTONES??!

The nucleosome is the basic structural subunit of chromatin, consisting of ~200 bp of DNA and an octamer of histone proteins. Histones are conserved DNA-binding proteins that form the basic subunit of chromatin in eukaryotes. Histones H2A, H2B, H3, H4 form an octameric core around which DNA coils to form a nucleosome. Histone H1 is external to the nucleosome. A nonhistone is any structural…

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VolitionRx CEO Cameron Reynolds to Present Informational Webinar

Presentation and Live Q&A on Wednesday, May 20 at 2pm US EDT

NAMUR, Belgium, May 18, 2015 /PRNewswire/ – VolitionRx Limited (NYSE MKT: VNRX), a life sciences company focused on developing blood-based diagnostic tests for a broad range of cancer types and other conditions, today announced that its Chief Executive Officer, Cameron Reynolds, is scheduled to present an informational webcast, being held May 20, to provide interested parties with information on VolitionRx and its clinical programs assessing its blood-based Nucleosomics® technology platform for the detection of the most prevalent cancers.

During the webcast Mr. Reynolds will outline the importance of early cancer detection, differentiators of VolitionRx’s diagnostics platform, and how VolitionRx’s NuQ® tests are addressing the global need for improved, non-invasive, accurate, and cost-effective cancer diagnostics. A live question and answer session will follow the presentation.

Details of the webcast are as follows:

Participants can reserve their spot by registering at http://ift.tt/1AfQVbs. Participants unable to join can register and will be sent the recording.

About VolitionRx

VolitionRx is a life sciences company focused on developing blood-based diagnostic tests for different types of cancer. The tests are based on the science of Nucleosomics which is the practice of identifying and measuring nucleosomes in the bloodstream - an indication that cancer is present.

VolitionRx’s goal is to make the tests as common and simple to use, for both patients and doctors, as existing diabetic and cholesterol blood tests. VolitionRx’s research and development activities are currently centred in Belgium as the company focuses on bringing its diagnostic products to market first in Europe, then in the US and ultimately, worldwide.

Visit VolitionRx’s website (www.volitionrx.com) or connect with us on Twitter, LinkedIn, Facebook or YouTube.

Media Contacts

Charlotte Reynolds, VolitionRx
Charlotte.Reynolds@volitionrx.com
Telephone: +44 (0) 795 217 7498

Kirsten Thomas, The Ruth Group
kthomas@theruthgroup.com
Telephone: +1 (646) 536-7014

Investor Contacts

Scott Powell, VolitionRx
S.Powell@volitionrx.com
Telephone: +1 (646) 650-1351

Lee Roth, The Ruth Group
lroth@theruthgroup.com
Telephone: +1 (646) 536-7012

Safe Harbor Statement

Statements in this press release may be “forward-looking statements” within the meaning of Section 27A of the Securities Act of 1933, as amended, and Section 21E of the Securities Exchange Act of 1934, as amended, that concern matters that involve risks and uncertainties that could cause actual results to differ materially from those anticipated or projected in the forward-looking statements. Words such as “expects,” “anticipates,” “intends,” “plans,” “believes,” “seeks,” “estimates,” “optimizing,” “potential,” “goal,” “suggests” and similar expressions identify forward-looking statements. These forward-looking statements relate to the effectiveness of the Company’s blood-based diagnostic test as well as the Company’s ability to develop and successfully commercialize such test platform for early detection of cancer. The Company’s actual results may differ materially from those indicated in these forward-looking statements due to numerous risks and uncertainties. For instance, if we fail to develop and commercialize diagnostic products, we may be unable to execute our plan of operations. Other risks and uncertainties include the Company’s failure to obtain necessary regulatory clearances or approvals to distribute and market future products in the clinical IVD market; a failure by the marketplace to accept the products in the Company’s development pipeline or any other diagnostic products the Company might develop; the Company will face fierce competition and the Company’s intended products may become obsolete due to the highly competitive nature of the diagnostics market and its rapid technological change; and other risks identified in the Company’s most recent Annual Report on Form 10-K and Quarterly Report on Form 10-Q, as well as other documents that the Company files with the Securities and Exchange Commission. These statements are based on current expectations, estimates and projections about the Company’s business based, in part, on assumptions made by management. These statements are not guarantees of future performance and involve risks, uncertainties and assumptions that are difficult to predict. Forward-looking statements are made as of the date of this release, and, except as required by law, the Company does not undertake an obligation to update its forward-looking statements to reflect future events or circumstances.

To view the original version on PR Newswire, visit:http://ift.tt/1L1aswN



Read this news on PR Newswire Asia website: VolitionRx CEO Cameron Reynolds to Present Informational Webinar
VolitionRx CEO Cameron Reynolds to Present Informational Webinar

Presentation and Live Q&A on Wednesday, May 20 at 2pm US EDT

NAMUR, Belgium, May 18, 2015 /PRNewswire/ – VolitionRx Limited (NYSE MKT: VNRX), a life sciences company focused on developing blood-based diagnostic tests for a broad range of cancer types and other conditions, today announced that its Chief Executive Officer, Cameron Reynolds, is scheduled to present an informational webcast, being held May 20, to provide interested parties with information on VolitionRx and its clinical programs assessing its blood-based Nucleosomics® technology platform for the detection of the most prevalent cancers.

During the webcast Mr. Reynolds will outline the importance of early cancer detection, differentiators of VolitionRx’s diagnostics platform, and how VolitionRx’s NuQ® tests are addressing the global need for improved, non-invasive, accurate, and cost-effective cancer diagnostics. A live question and answer session will follow the presentation.

Details of the webcast are as follows:

Participants can reserve their spot by registering at http://ift.tt/1AfQVbs. Participants unable to join can register and will be sent the recording.

About VolitionRx

VolitionRx is a life sciences company focused on developing blood-based diagnostic tests for different types of cancer. The tests are based on the science of Nucleosomics which is the practice of identifying and measuring nucleosomes in the bloodstream - an indication that cancer is present.

VolitionRx’s goal is to make the tests as common and simple to use, for both patients and doctors, as existing diabetic and cholesterol blood tests. VolitionRx’s research and development activities are currently centred in Belgium as the company focuses on bringing its diagnostic products to market first in Europe, then in the US and ultimately, worldwide.

Visit VolitionRx’s website (www.volitionrx.com) or connect with us on Twitter, LinkedIn, Facebook or YouTube.

Media Contacts

Charlotte Reynolds, VolitionRx
Charlotte.Reynolds@volitionrx.com
Telephone: +44 (0) 795 217 7498

Kirsten Thomas, The Ruth Group
kthomas@theruthgroup.com
Telephone: +1 (646) 536-7014

Investor Contacts

Scott Powell, VolitionRx
S.Powell@volitionrx.com
Telephone: +1 (646) 650-1351

Lee Roth, The Ruth Group
lroth@theruthgroup.com
Telephone: +1 (646) 536-7012

Safe Harbor Statement

Statements in this press release may be “forward-looking statements” within the meaning of Section 27A of the Securities Act of 1933, as amended, and Section 21E of the Securities Exchange Act of 1934, as amended, that concern matters that involve risks and uncertainties that could cause actual results to differ materially from those anticipated or projected in the forward-looking statements. Words such as “expects,” “anticipates,” “intends,” “plans,” “believes,” “seeks,” “estimates,” “optimizing,” “potential,” “goal,” “suggests” and similar expressions identify forward-looking statements. These forward-looking statements relate to the effectiveness of the Company’s blood-based diagnostic test as well as the Company’s ability to develop and successfully commercialize such test platform for early detection of cancer. The Company’s actual results may differ materially from those indicated in these forward-looking statements due to numerous risks and uncertainties. For instance, if we fail to develop and commercialize diagnostic products, we may be unable to execute our plan of operations. Other risks and uncertainties include the Company’s failure to obtain necessary regulatory clearances or approvals to distribute and market future products in the clinical IVD market; a failure by the marketplace to accept the products in the Company’s development pipeline or any other diagnostic products the Company might develop; the Company will face fierce competition and the Company’s intended products may become obsolete due to the highly competitive nature of the diagnostics market and its rapid technological change; and other risks identified in the Company’s most recent Annual Report on Form 10-K and Quarterly Report on Form 10-Q, as well as other documents that the Company files with the Securities and Exchange Commission. These statements are based on current expectations, estimates and projections about the Company’s business based, in part, on assumptions made by management. These statements are not guarantees of future performance and involve risks, uncertainties and assumptions that are difficult to predict. Forward-looking statements are made as of the date of this release, and, except as required by law, the Company does not undertake an obligation to update its forward-looking statements to reflect future events or circumstances.

To view the original version on PR Newswire, visit:http://ift.tt/1L1aswN



Read this news on PR Newswire Asia Website: VolitionRx CEO Cameron Reynolds to Present Informational Webinar
Study evaluates VolitionRx’s proprietary Nucleosomics platform in most prevalent cancers

VolitionRx Limited (NYSE MKT: VNRX), a life sciences company focused on developing blood-based diagnostic tests for a broad range of cancer types and other conditions, today announced that it has accelerated its large independent prospective study evaluating its proprietary Nucleosomics® …
Source:Study evaluates VolitionRx’s proprietary Nucleosomics platform in most prevalent cancers

The role of epigenetics in personalized medicine: challenges and opportunities.

PubMed: The role of epigenetics in personalized medicine: challenges and opportunities.

BMC Med Genomics. 2015 Jan 15;8 Suppl 1:S5

Authors: Rasool M, Malik A, Naseer MI, Manan A, Ansari S, Begum I, Qazi MH, Pushparaj P, Abuzenadah AM, Al-Qahtani MH, Kamal MA, Gan S

Abstract
Epigenetic alterations are considered to be very influential in both the normal and disease states of an organism. These alterations include methylation, acetylation, phosphorylation, and ubiquitylation of DNA and histone proteins (nucleosomes) as well as chromatin remodeling. Many diseases, such as cancers and neurodegenerative disorders, are often associated with epigenetic alterations. DNA methylation is one important modification that leads to disease. Standard therapies are given to patients; however, few patients respond to these drugs, because of various molecular alterations in their cells, which may be partially due to genetic heterogeneity and epigenetic alterations. To realize the promise of personalized medicine, both genetic and epigenetic diagnostic testing will be required. This review will discuss the advances that have been made as well as the challenges for the future.

PMID: 25951941 [PubMed - in process] http://dlvr.it/9l9MGY

VolitionRx Accelerates Clinical Trial with University Hospital Bonn, Germany Evaluating NuQ(R) Assays in Most Prevalent Cancers

Study expanded to evaluate VolitionRx’s proprietary Nucleosomics? platform in 4,200 patient samples across 27 of the most prevalent cancers

Sample collection nearly complete, accelerated analysis to begin in early Q3 2015 with dedication of two automated robots

NAMUR, Belgium, May 8, 2015 /PRNewswire/ – VolitionRx Limited (NYSE MKT: VNRX), a life sciences company focused on developing blood-based diagnostic tests for a broad range of cancer types and other conditions, today announced that it has accelerated its large independent prospective study evaluating its proprietary Nucleosomics® platform at University Hospital Bonn in Germany. VolitionRx has dedicated two of its recently-procured Tecan EVO200 automated laboratory robots to the study, with sample analysis to begin early in the third quarter of 2015. In addition, VolitionRx and study lead Prof. Stefan Holdenrieder at the Institute of Clinical Chemistry and Clinical Pharmacology at University Hospital Bonn in Germany have increased the sample size and breadth of cancer types being tested.

The study initially included 4,000 patient blood samples across 20 of the most prevalent cancer types, and has now been expanded to include approximately 4,200-patient blood samples across 27 of the most prevalent cancers, including: two types of respiratory cancer, seven types of gastrointestinal cancer, four gynecological cancers, four urinary cancers, four types of hematological cancer, plus melanoma, sarcoma and cancers of the thyroid and brain; as well as control patients with 24 other conditions and healthy individuals. In total, the cancer types being analyzed represent over 95% of the incidence of cancer.

Dr. Jake Micallef, VolitionRx’s Chief Scientific Officer, said, “This trial is an incredibly important one, which we hope will show the breadth of our Nucleosomics® technology. The trial is not only essentially 27 pilot studies in the most common cancers, but we are also looking for differences between cancers, and between cancers and other diseases, to discover if a panel of assays can detect several cancers from the same blood draw. We are grateful to Prof. Holdenrieder for his commitment to this ongoing multi-year trial, demonstrated by the increase of sample size and broadening of cancer types being evaluated in the trial - both of which we hope will enable us to more clearly demonstrate accuracy rates of our NuQ® test in these patient populations.”

Cameron Reynolds, Chief Executive Officer of VolitionRx, commented, “We are very excited that with the addition of two fully-operational automated robot systems, analysis of the full 4,200 sample collection is on track to begin early in the third quarter of 2015. We expect to analyze the samples with up to 10-15 NuQ® assays by the first quarter of 2016. The aim of the trial is both to evaluate a large number of cancers and to look for a panel of assays that can diagnose multiple cancers for potential new products beyond the current four cancers we are evaluating.”

Prof. Stefan Holdenrieder at the Institute of Clinical Chemistry and Clinical Pharmacology at University Hospital Bonn in Germany, who is leading the study, added, “Collectively, cancer is one of the foremost causes of mortality around the world and there remains a significant global need for improved early cancer detection. An accurate and non-invasive test using only a drop of blood that could detect a range of cancers would provide physicians and patients an opportunity to treat the disease early on, before the cancer metastasizes, providing greater chances of survival. Collection and analysis of samples has been, and will continue to be, carried out in my laboratory with VolitionRx providing the assays for analysis. I am very much looking forward to seeing the results from this study.”

The NuQ® tests utilize the Company’s proprietary Nucleosomics® technology platform, which identifies and measures circulating nucleosome structures for the presence of epigenetic cancer and signals within the blood.

Clinical trials assessing the effectiveness of VolitionRx’s assays include:

Colorectal cancer:

  • A 4,800 patient retrospective symptomatic population study (Hvidovre Hospital, University of Copenhagen, Denmark)
  • A 14,000 patient prospective screening study (Hvidovre Hospital, University of Copenhagen, Denmark)
  • A 250 patient prospective study (CHU-UCL Mont Godinne Hospital, Belgium)

Pre-cancerous colorectal adenomas:

  • A 800 patient prospective study (Hvidovre Hospital, University of Copenhagen, Denmark)

27 most prevalent cancers

  • A 4,200 patient prospective study that involves patients with the 27 most prevalent cancers (University Hospital, Bonn, Germany)

Lung cancer:

  • A 600 patient prospective confirmatory study (University Hospital, Bonn, Germany)

Prostate cancer:

  • A retrospective study to establish the efficacy of VolitionRx’s NuQ® tests to distinguish anaplastic prostate cancer, a particularly aggressive form of the disease, from typical castration resistant prostate cancer (CRPC), the less aggressive form (MD Anderson Cancer Center, Texas)
  • A 120 patient prospective feasibility study (ImmuneHealth, Belgium)

Ovarian cancer:

  • A 40 patient prospective feasibility study (Singapore General Hospital, Singapore)

Endometriosis

  • A prospective study to assess VolitionRx’s NuQ® tests for the diagnosis of endometriosis (the University of Oxford, United Kingdom)

About VolitionRx

VolitionRx is a life sciences company focused on developing diagnostic tests for cancer and other conditions. The tests are based on the science of Nucleosomics®, which is the practice of identifying and measuring nucleosomes in the bloodstream or other bodily fluid - an indication that disease is present.

VolitionRx’s goal is to make the tests as common and simple to use, for both patients and doctors, as existing diabetic and cholesterol blood tests. VolitionRx’s research and development activities are currently centered in Belgium as the company focuses on bringing its diagnostic products to market first in Europe, then in the US and ultimately, worldwide.

Visit VolitionRx’s website (http://ift.tt/V4ODcg) or connect with us via Twitter, LinkedIn, Facebook or YouTube.

Media Contacts

Charlotte Reynolds, VolitionRx
Charlotte.Reynolds@volitionrx.com
Telephone: +44 (0) 795 217 7498

Kirsten Thomas, The Ruth Group
kthomas@theruthgroup.com
Telephone: +1 (646) 536-7014

Investor Contacts

Scott Powell, VolitionRx
S.Powell@volitionrx.com
Telephone: +1 (646) 650-1351

Lee Roth, The Ruth Group
lroth@theruthgroup.com
Telephone: +1 (646) 536-7012

Safe Harbor Statement

Statements in this press release may be “forward-looking statements” within the meaning of Section 27A of the Securities Act of 1933, as amended, and Section 21E of the Securities Exchange Act of 1934, as amended, that concern matters that involve risks and uncertainties that could cause actual results to differ materially from those anticipated or projected in the forward-looking statements. Words such as “expects,” “anticipates,” “intends,” “plans,” “believes,” “seeks,” “estimates,” “optimizing,” “potential,” “goal,” “suggests” and similar expressions identify forward-looking statements. These forward-looking statements relate to the effectiveness of the Company’s bodily-fluid-based diagnostic tests as well as the Company’s ability to develop and successfully commercialize such test platforms for early detection of cancer. The Company’s actual results may differ materially from those indicated in these forward-looking statements due to numerous risks and uncertainties. For instance, if we fail to develop and commercialize diagnostic products, we may be unable to execute our plan of operations. Other risks and uncertainties include the Company’s failure to obtain necessary regulatory clearances or approvals to distribute and market future products in the clinical IVD market; a failure by the marketplace to accept the products in the Company’s development pipeline or any other diagnostic products the Company might develop; the Company will face fierce competition and the Company’s intended products may become obsolete due to the highly competitive nature of the diagnostics market and its rapid technological change; and other risks identified in the Company’s most recent Annual Report on Form 10-K and Quarterly Report on Form 10-Q, as well as other documents that the Company files with the Securities and Exchange Commission. These statements are based on current expectations, estimates and projections about the Company’s business based, in part, on assumptions made by management. These statements are not guarantees of future performance and involve risks, uncertainties and assumptions that are difficult to predict. Forward-looking statements are made as of the date of this release, and, except as required by law, the Company does not undertake an obligation to update its forward-looking statements to reflect future events or circumstances.

To view the original version on PR Newswire, visit:http://ift.tt/1H3vslS



Read this news on PR Newswire Asia Website: VolitionRx Accelerates Clinical Trial with University Hospital Bonn, Germany Evaluating NuQ® Assays in Most Prevalent Cancers
VolitionRx Accelerates Clinical Trial with University Hospital Bonn, Germany Evaluating NuQ(R) Assays in Most Prevalent Cancers

Study expanded to evaluate VolitionRx’s proprietary Nucleosomics? platform in 4,200 patient samples across 27 of the most prevalent cancers

Sample collection nearly complete, accelerated analysis to begin in early Q3 2015 with dedication of two automated robots

NAMUR, Belgium, May 8, 2015 /PRNewswire/ – VolitionRx Limited (NYSE MKT: VNRX), a life sciences company focused on developing blood-based diagnostic tests for a broad range of cancer types and other conditions, today announced that it has accelerated its large independent prospective study evaluating its proprietary Nucleosomics® platform at University Hospital Bonn in Germany. VolitionRx has dedicated two of its recently-procured Tecan EVO200 automated laboratory robots to the study, with sample analysis to begin early in the third quarter of 2015. In addition, VolitionRx and study lead Prof. Stefan Holdenrieder at the Institute of Clinical Chemistry and Clinical Pharmacology at University Hospital Bonn in Germany have increased the sample size and breadth of cancer types being tested.

The study initially included 4,000 patient blood samples across 20 of the most prevalent cancer types, and has now been expanded to include approximately 4,200-patient blood samples across 27 of the most prevalent cancers, including: two types of respiratory cancer, seven types of gastrointestinal cancer, four gynecological cancers, four urinary cancers, four types of hematological cancer, plus melanoma, sarcoma and cancers of the thyroid and brain; as well as control patients with 24 other conditions and healthy individuals. In total, the cancer types being analyzed represent over 95% of the incidence of cancer.

Dr. Jake Micallef, VolitionRx’s Chief Scientific Officer, said, “This trial is an incredibly important one, which we hope will show the breadth of our Nucleosomics® technology. The trial is not only essentially 27 pilot studies in the most common cancers, but we are also looking for differences between cancers, and between cancers and other diseases, to discover if a panel of assays can detect several cancers from the same blood draw. We are grateful to Prof. Holdenrieder for his commitment to this ongoing multi-year trial, demonstrated by the increase of sample size and broadening of cancer types being evaluated in the trial - both of which we hope will enable us to more clearly demonstrate accuracy rates of our NuQ® test in these patient populations.”

Cameron Reynolds, Chief Executive Officer of VolitionRx, commented, “We are very excited that with the addition of two fully-operational automated robot systems, analysis of the full 4,200 sample collection is on track to begin early in the third quarter of 2015. We expect to analyze the samples with up to 10-15 NuQ® assays by the first quarter of 2016. The aim of the trial is both to evaluate a large number of cancers and to look for a panel of assays that can diagnose multiple cancers for potential new products beyond the current four cancers we are evaluating.”

Prof. Stefan Holdenrieder at the Institute of Clinical Chemistry and Clinical Pharmacology at University Hospital Bonn in Germany, who is leading the study, added, “Collectively, cancer is one of the foremost causes of mortality around the world and there remains a significant global need for improved early cancer detection. An accurate and non-invasive test using only a drop of blood that could detect a range of cancers would provide physicians and patients an opportunity to treat the disease early on, before the cancer metastasizes, providing greater chances of survival. Collection and analysis of samples has been, and will continue to be, carried out in my laboratory with VolitionRx providing the assays for analysis. I am very much looking forward to seeing the results from this study.”

The NuQ® tests utilize the Company’s proprietary Nucleosomics® technology platform, which identifies and measures circulating nucleosome structures for the presence of epigenetic cancer and signals within the blood.

Clinical trials assessing the effectiveness of VolitionRx’s assays include:

Colorectal cancer:

  • A 4,800 patient retrospective symptomatic population study (Hvidovre Hospital, University of Copenhagen, Denmark)
  • A 14,000 patient prospective screening study (Hvidovre Hospital, University of Copenhagen, Denmark)
  • A 250 patient prospective study (CHU-UCL Mont Godinne Hospital, Belgium)

Pre-cancerous colorectal adenomas:

  • A 800 patient prospective study (Hvidovre Hospital, University of Copenhagen, Denmark)

27 most prevalent cancers

  • A 4,200 patient prospective study that involves patients with the 27 most prevalent cancers (University Hospital, Bonn, Germany)

Lung cancer:

  • A 600 patient prospective confirmatory study (University Hospital, Bonn, Germany)

Prostate cancer:

  • A retrospective study to establish the efficacy of VolitionRx’s NuQ® tests to distinguish anaplastic prostate cancer, a particularly aggressive form of the disease, from typical castration resistant prostate cancer (CRPC), the less aggressive form (MD Anderson Cancer Center, Texas)
  • A 120 patient prospective feasibility study (ImmuneHealth, Belgium)

Ovarian cancer:

  • A 40 patient prospective feasibility study (Singapore General Hospital, Singapore)

Endometriosis

  • A prospective study to assess VolitionRx’s NuQ® tests for the diagnosis of endometriosis (the University of Oxford, United Kingdom)

About VolitionRx

VolitionRx is a life sciences company focused on developing diagnostic tests for cancer and other conditions. The tests are based on the science of Nucleosomics®, which is the practice of identifying and measuring nucleosomes in the bloodstream or other bodily fluid - an indication that disease is present.

VolitionRx’s goal is to make the tests as common and simple to use, for both patients and doctors, as existing diabetic and cholesterol blood tests. VolitionRx’s research and development activities are currently centered in Belgium as the company focuses on bringing its diagnostic products to market first in Europe, then in the US and ultimately, worldwide.

Visit VolitionRx’s website (http://ift.tt/V4ODcg) or connect with us via Twitter, LinkedIn, Facebook or YouTube.

Media Contacts

Charlotte Reynolds, VolitionRx
Charlotte.Reynolds@volitionrx.com
Telephone: +44 (0) 795 217 7498

Kirsten Thomas, The Ruth Group
kthomas@theruthgroup.com
Telephone: +1 (646) 536-7014

Investor Contacts

Scott Powell, VolitionRx
S.Powell@volitionrx.com
Telephone: +1 (646) 650-1351

Lee Roth, The Ruth Group
lroth@theruthgroup.com
Telephone: +1 (646) 536-7012

Safe Harbor Statement

Statements in this press release may be “forward-looking statements” within the meaning of Section 27A of the Securities Act of 1933, as amended, and Section 21E of the Securities Exchange Act of 1934, as amended, that concern matters that involve risks and uncertainties that could cause actual results to differ materially from those anticipated or projected in the forward-looking statements. Words such as “expects,” “anticipates,” “intends,” “plans,” “believes,” “seeks,” “estimates,” “optimizing,” “potential,” “goal,” “suggests” and similar expressions identify forward-looking statements. These forward-looking statements relate to the effectiveness of the Company’s bodily-fluid-based diagnostic tests as well as the Company’s ability to develop and successfully commercialize such test platforms for early detection of cancer. The Company’s actual results may differ materially from those indicated in these forward-looking statements due to numerous risks and uncertainties. For instance, if we fail to develop and commercialize diagnostic products, we may be unable to execute our plan of operations. Other risks and uncertainties include the Company’s failure to obtain necessary regulatory clearances or approvals to distribute and market future products in the clinical IVD market; a failure by the marketplace to accept the products in the Company’s development pipeline or any other diagnostic products the Company might develop; the Company will face fierce competition and the Company’s intended products may become obsolete due to the highly competitive nature of the diagnostics market and its rapid technological change; and other risks identified in the Company’s most recent Annual Report on Form 10-K and Quarterly Report on Form 10-Q, as well as other documents that the Company files with the Securities and Exchange Commission. These statements are based on current expectations, estimates and projections about the Company’s business based, in part, on assumptions made by management. These statements are not guarantees of future performance and involve risks, uncertainties and assumptions that are difficult to predict. Forward-looking statements are made as of the date of this release, and, except as required by law, the Company does not undertake an obligation to update its forward-looking statements to reflect future events or circumstances.

To view the original version on PR Newswire, visit:http://ift.tt/1H3vslS



Read this news on PR Newswire Asia website: VolitionRx Accelerates Clinical Trial with University Hospital Bonn, Germany Evaluating NuQ® Assays in Most Prevalent Cancers