myotubes

Myogenesis

[how mature muscle cells are made]

Muscle fibers generally form the fusion of myoblasts into multi-nucleated fibers called myotubes. 

  • In early development, myoblasts can either proliferate, or differentiate into a myotube. 
  • once differentiated, myoblasts align 
  • cells fuse -  presence of calcium ions is critical, involves recruitment of actin to the plasma membrane, followed by close apposition and creation of a pore that subsequently rapidly widens.

Stages and associated genetic factors

  • Delamination –> PAX3, c-Met
  • Migration –> c-met/HGF, LBX1
  • Proliferation –> PAX3, c-Met, Mox2, MSX1, Six1/4, Myf5, MyoD
  • Determination –>Myf5 and MyoD
  • Differentiation –> Myogenin, MCF2, Six1/4, MyoD, Myf6
  • Specific Muscle Formation –> Lbx1, Meox2
  • Satellite Cells –> PAX7

I’m back, folks !

Like I said, I got an internship in a lab, I spent two months there doing cell cultures, fluorescence microscopy and a lot of reading. Despite my heart made of stone, I got the urge to cry twice, when I first used the confocal microscope, and when I left it. Actually no, I also almost cried out of frustration when the Leica TCS SP2 bugged out on us and would not work until the end of my internship. Fortunately, we had only a week without a microscope, until we switched to the Olympus FV-1000.

I can’t actually show you any of my work because it’s not published yet. Depending on when it’s actually submitted and when (if) it’s accepted, it might take a while before I can show my beauties. They were C2C12 (muscle) cells, like the ones above, and although they died on me quite a lot, they were a joy to hang around with. 

This was Frontal Cortex, anthropomorphising cells since forever.

See you !