I managed to capture the exact moment Jason loses all hope at the asylum the last time I played Arkham Knight. This is just after Joker shows him the picture of Bruce and Tim, a bit after Jason says that pitiful “no.” Before this, you could see a lot more emotion expressed on his face – anger, sadness. Now his face is blank, overwhelmed by sadness and anger and disappointment and fear. Because this is the exact point that he realizes that nobody’s coming to save him.
If you look closely, you can see tears welling up in his eyes.