Sink used for Acid Fast Bacilli Kinyoun stain. This is at the lab I’m currently doing my rotations at and I thought it was pretty.
Mycobacterium tuberculosis, the causative agent for Tuberculosis or TB, has a cell wall that’s high in lipid content. So, it doesn’t stain with regular gram stain; thus, they used acid fast staining techniques with carbolfuchsin.
I work in a hospital microbiology lab! We get all sorts of specimens ranging from urine and stool to blood, sputum, sterile body fluids (like synovial fluid), cerebral spinal fluid, and tissue. We do pathogen identification and antibiotic susceptibility testing.
The patient from the post has what is most likely going to be a staph infection, based on the preliminary Gram stain.
Pseudomonas aeruginosa is a nonfermentative gram-negative bacillus. This bacterium is ubiquitous in soil and water, and on surfaces in contact with soil or water. Pseudomonas aeruginosa is an opportunistic pathogen, meaning that it exploits some break in the host defenses to initiate an infection. It causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections and a variety of systemic infections, particularly in patients with severe burns and in cancer and AIDS patients who are immunosuppressed.
Key characteristics: Gram(-), catalase(+), oxidase(+), motility(+), aerobic, produces pigments (green and blue), grows at 42°C.
I'm doing a gram stain at home and I was wondering if it was safe (for a septic system) to wash the liquid down the drain. Thanks in advance.
i really don’t know about a septic system - i do know that there’s an eco version so i’d probably look into that if i were you as the fact it exists probably means there’s a reason for it (saying that, i don’t think one or even a handful of stains will be a problem just don’t do hundreds until you know for sure - far worse gets put down household drains)
S. aureus is a type of bacteria. It stains Gram positive non-motile cocci. It is found in grape-like clusters. This is why it is called Staphylococcus.
Staphylococcus is one of the five most common causes of infections after injury or surgery. It affects around 500,000 patients in American hospitals annually.
S. aureus may occur commonly in the environment. S. aureus is transmitted through air droplets or aerosol. Another common method of transmission is through direct contact with objects that are contaminated by the bacteria or by bites from infected persons or animals. Approximately 30% of healthy humans carry S. aureus in their nose, back of the throat and on their skin.
S. aureus has long been recognized as one of the most important bacteria that cause disease in humans. It is the leading cause of skin and soft tissue infections such as abscesses (boils), furuncles, and cellulitis. Although most staph infections are not serious, S. aureus can cause serious infections such as bloodstream infections, pneumonia, or bone and joint infections.
When S. aureus becomes resistant to many of the antibiotics used to treat ordinary infections we call it Methicillin-resistant Staphylococcus aureus (MRSA).
I volunteered in January to be a part of this “Science Event” development team to work on the Microbiology part. Two of my peers and I were the helpers. I had a lot of fun. Don’t get me wrong, it was incredibly stressful and I think I scared both of my collaborators when my finely hidden enthusiasm for microbiology emerged. I get much too excited over these things. I mean, I think about it a lot. I can’t lie. I’ve wanted to work with diseases and causative agents since I was in middle school and this desire has not waned. It grew. Lovely. But I hide my excitement…by being very sedate and trying not to look at things too long.
But anyway, I did a lot of work for this. I guess it was a mixed blessing/curse not having my regular micro job last week…for one, I got rather depressed not having any micro to do, but I found out rather quickly that I had more to do than I had originally thought. So, more time ftw!
The three of us actually pieced everything together. I mean everything! We didn’t steal any questions. We read everything that the teams were supposed to know. We went around getting the samples we’d need. We rewrote questions to fit what we could obtain. We fixed questions last minute to make way for plans that had fallen through.
I personally, proofread our questions, made an answer key and an answer sheet.
Fun story! We needed a slide of Gram + bacteria (Gram stained purple). We asked the head of the event at our school, but she doesn’t deal with bacteria, so she told us to ask our Microbiology teacher. However, he was out of town. So, one of us (not me) emailed him to check if he may have any prepared slides we could use. But then we got word that our specific adviser for the micro event had prepared slides, so we were relieved. But the day of, we discovered that her prepared slides were really old and fading and all the Gram + Micrococcus luteus looked red. (how confusing). And then we heard from the head that she had talked to our Micro teacher who was going to make us Gram stains. But was told we already had them. Damn it! *Note: I thought that the gesture to make us the Gram stains was very kind.
So, we got into the Micro lab and I made the Gram stain with old M. luteus which turned out a lot better than I expected. The adviser said it was perfectly fine. When we took it to another microscope, I liked it better.
Anyway, I think we made the test too hard even by following the guidelines for what they should have known. I can’t say anything specific and I won’t. Other than the Gram stain part.
There’s Pepper getting weighed. He’s lost weight it would seem. They examined him, did a crop swab, and a gram stain.
The verdict? Well, Pepper has a bacterial infection. There was a lot of gram negative bacteria in his crop. They took blood and are doing a CBC to look for underlying causes. The results of that will likely be told to me Monday.
In the meantime, Pepper needs to be given Baytril, an antibiotic, every 12 hours, followed by Benebac Plus. Depending on the results of the blood test, and if the antibiotics help him recover, he may need to go back in.
Thank you so much again for your generosity! I was able to cover the entire vet bill, and should be able to cover the next visit if he needs to return. Any leftover money is going in a savings account to be used in the event of another bird emergency.
johnlock au where john and sherlock are microbes. johns a gram negative bacterial cell bc he has lots of layers like an ogre, while sherlock is a gram positive cell bc he has one big thick dfp barrier between his literally microscopic smoll self and the world (also he stains purple during a gram stain, so like his purple shirt 💜). john tries to touch sherlock w his big long monster sex pilus to ~exchange genetic information~ sherlock does a happy wiggle when it touches his cell butt
So unfortunately I am at the vet right now. Poor Brienne finch is not doing well at all. Right now they are doing a gram stain test to see if they can find anything. They want to do an X-ray, but she is already stressed and the vet is worried the stress from an x-ray would kill her. I’ll keep you guys updated. Send good thoughts to wee finch.