Sirius Black waking up in the middle of the night thinking he hears hooves but it’s really the sound of a metal goblet banging against the cell doors.
Prongs would never ride again.
Sirius Black waking up in the middle of the night because the full moon is too bright and all he can think about is Remus, alone again.
Moony would never have the Marauders again.
Sirius Black waking up in the middle of the night because a something furry brushes by him and he shoots up, lunging across his cell for it until he remembers Peter is running free when he is not.
Wormtail would never show his face again.
Sirius Black, sleep deprived and isolated, wrongly imprisoned for a crime he wouldn’t even dream to commit. His life a walking nightmare filled with the friends he’d never see again and the voices he’d kill to hear one last time. Sirius Black, aging and lifeless, counting down the days until he was free, in this life or in the next.
CFTR (cystic fibrosis transmembrane conductance regulator) protein expression in well-differentiated human bronchial epithelial cultures. Well-differentiated cultures derived from human bronchial epithelial tissues were immunostained with CFTR and tubulin antibodies and analyzed on a Leica SP2 laser confocal microscope. The image represents an overlay of the DIC (grayscale), CFTR (red), cilia (tubulin, green), and nuclei (DAPI, blue) confocal planes, and depicts an epithelial cell sheet that contains a group of ciliated cells surrounding a goblet cell (bottle-shaped cell with no cilia). CFTR is expressed only at the apical membrane of ciliated cells, but not goblet cells. Magnification x190. Reproduced from Kreda et al 2005 Miol Biol Cell 16, 2154 with permission of ASCB / MBC.
The CFTR protein functions as a channel across the membrane of cells that produce mucus, sweat, saliva, tears, and digestive enzymes. The channel transports chloride ions into and out of cells. The transport of chloride ions helps control the movement of water in tissues, which is necessary for the production of thin, freely flowing mucus. Mucus is a slippery substance that lubricates and protects the lining of the airways, digestive system, reproductive system, and other organs and tissues.
More than 1,000 mutations in the CFTR gene have been identified in people with cystic fibrosis. The resulting abnormal channel breaks down shortly after it is made, so it never reaches the cell membrane to transport chloride ions and the movement of water into and out of cells.
As a result, cells that line the passageways of the lungs, pancreas, and other organs produce mucus that is abnormally thick and sticky. The abnormal mucus obstructs the airways and glands, leading to the characteristic signs and symptoms of cystic fibrosis.
Microscopically, the mucosal lining of the colon exhibits many intestinal glands called crypts
of Lieberkuhn (flowers) that are lined with mucus secreting
goblet cells (petals).
Mucus collects in the lumen of the gland (center of flower) and is
squeezed out when the muscle of your colon contracts to push the fecal
material towards the rectum. As the feces move through the colon water
is reabsorbed making them drier to conserve the water. As a result, the
mucus secreted from the crypts is super important in this region because
it ensures that the tube is well lubricated to allow the dry feces to
be passed along the colon easily without causing any damage (the mucus
If you slice up the colon in a way that
ensures you cut through the mucosa parallel with its
surface and then stain it using dyes that show the mucus inside the
goblet cells (in this case HEA50). Then, your colon looks much more like
a field full of daisies with pretty goblet cell petals than a chute for
passing along poop.
Histology 📷 from the microscope of the ever fragrant @NejibY (Insta)
Be warned however, this is one posy that you really don’t want to sniff.
Microscopically, the mucosal lining of the colon exhibits many crypts of Lieberkuhn (intestinal glands) that are lined with mucus secreting goblet cells.
Mucus is useful in this region because it assists in lubricating the tube to allow dry feces to be passed along easily (decreases friction).
When the a slice is made through the mucosa parallel with its orientation and stained specifically for mucin, these crypts glands look remarkably like a field full of daisies. The goblet cells forming the petals.