genome-project

Human DNA: A history

DNA is an important part of modern crime evidence, but it is important to know when DNA is actually used in history, and the experiments it took to actually get DNA to be the robust science it is today. Hopefully this is helpful for your writing needs!


DNA Timeline

1866 – Gregor Mendel determines the principles of genetic inheritance

1902 – Sir Archibald Edward Garrod associated Mendel’s theories with human disease alkaptonuria (black urine or black bone disease, genetic disorder where the body cannot process the amino acids phenylalanine and tyrosine)

1944 – Oswald Avery, working with MacLeod and McCarty determines DNA as the “transforming principle”, while working with Streptococcus pneumoniae (bacteria causing pneumonia), it was determined that dead/heat-killed S-strain (which is lethal) can “transform” living R-strain (which is non-lethal) into lethal bacteria

1950 - Chargaff’s rules was developed by Edwin Chargaff, it basically states that there is a 1:1 ratio of pyrimidine and purine bases, that adenine = thymine, and guanine = cytosine, an important rule for base pairings and the DNA double helix structure

1952 – Alfred Hershey and Martha Chase experimented with T2 phage and elemental isotopes; phosphorus-32 labelled DNA while sulfur-35 labelled the proteins; the progeny infected unlabelled bacteria, and since phosphorus-32 showed up, it confirmed that DNA is the genetic material

1952 – Rosalind Franklin uses X-ray crystallography to photograph DNA fibres

1953 – James Watson and Francis Crick uses Franklin’s crystallography and previous research material to determine the double helix structure of DNA

1959 – an extra chromosome 21 is linked to Down’s syndrome

1965 – Marshall Nirenberg worked with E.coli bacteria cells to determine how DNA is involved with RNA and protein expressions, he helped decipher the codons of the genetic code

1977 – Frederick Sanger develops a technique for rapid DNA sequencing, the Sanger sequencing is also known as chain termination method based on selectively incorporating chain-terminating dideoxynucleotides (ddNTPs)

1984 – Sir Alec Jeffreys developed the multi-locus RFLP probes, a method to compare the variations in DNA of different individuals, leading way to genetic fingerprinting

1986 – The first use of DNA testing in a forensic case, using Jeffreys’ multi-locus RFLP probes for DNA typing to catch Colin Pitchfork, who sexually assaulted and murdered Lunda Mann in 1983 and Dawn Ashworth in 1986

1990 – the Human Genome Project begins, with the goal of mapping all of the 3.3 billion bases of the human genome, and the genome of other organisms, this project costed about $2.7 billion (this amount wasn’t calculated for inflation) and took almost 15 years to complete, compare that to now where it will take about $1000 to sequence the human genome and it will take about a day to do so

2013 – it was discovered that identical (monozygotic) twins actually have differences in their genetic make-up, with differences/mutations called Single Nucleotide Polymorphism (SNPs)

You Scared of Me Now, Babydoll? (Part 9)

Pairing: Negan x Reader

Summary: You meet Eugene Porter when Negan brings him back to Sanctuary

Word Count: 1,899

Warning(s): Brief sexual content, Language

A/N: Yay for more scenes from the show! Masterlist here. Enjoy!

Originally posted by meanandshallow

You could feel warm kisses fluttering down your breasts. His peppered beard brushed against your skin, leaving it pink and flushed.

“Negan,” you pleaded, arching your back as his lips traveled further. He suckled down your abdomen and past your navel, lingering just above your pelvis. You moaned again. You could feel yourself drenched.

“Say my name one more time, babygirl. One more fuckin’ time and I’ll taste this pretty little pussy.”

He peered up at you, his eyes a deep rich mahogany. They were dark with desire and lust.

“Negan.”

Your voice came out as a moan and you could barely contain your composure.

His lips curled into a carnal smile.

Keep reading

TWD - Negan Imagine ~ "Hostiles and Calamities"

An imagine about the 11th episode of Season 7

I’m following the storyline of the TV show, so I wrote parts to the other episodes as well that are connected.
BUT they are all written the way that you can read them separate without having read any other part!

part 1: about the final scene of season 6
part 2: about the first episode of season 7
part 3: about the readers arrival in the sanctuary (for 7x02)
part 4: about 7x03 “The Cell”
part 5: about 7x04 “Service”
part 6: about 7x05 “Go Getters”
(7x06 was left out because the timeframe between the episodes was too short)
part 7: about 7x07 “Sing me a song”
part 8: about 7x08 the midseason finale “Hearts still beating”
part 9: about 7x09 “Rock in the Road”
(7x10 had to be left out)

Originally posted by dancing-at-the-funeralparty

The wind shook softly on the windows of your room while you looked at the swinging trees.
It was not too long ago that you came back from Alexandria, where you had to see a second time how the man who had taken you with him after the line up killed some of your people.
One of them completely innocent.
You had no idea where they had taken Eugene, you didn’t even know where your brother Daryl was after you had heard that he fled.
The only thing you knew was that he was probably not in Alexandria but you also knew that Negan and his men wouldnt stop searching for him and the thought of that still let a shiver run down your back.

You heard a knocking on the door and seconds after that Negan came smirking in, his bat laying on his shoulder.
“Good Morning Sweetheart. We two gotta do a lot stuff, meet some fucking people”, Negan said grinning as he came closer towards you.
“One of them is even a fucking old friend of yours. What was his name?”, he asked smirking.
“Eugene”, you hissed while Negan kept grinning at you.
“Where did you hold him?”, you asked glaring at him hoping that he wouldnt tell you that he was in one of those cells.
“No need to glare Sweetheart. He’s having a fucking nice ass apartment and I really do hope for him that he’s getting the mop on his head done right now and gets some food”, Negan said chuckling while he looked at you.
“You think I put him into one of the damn cells? No Sweetheart I need his damn brain to work intact”, he said chuckling before he continued.
“But apropos cells there is the dumbass we’re gonna meet before your mullet friend”, said Negan grinning before he walked towards you to get his arm around your waist while you tried to find out who Negan was talking about. “Would be impolite to let him wait, right?”, Negan said before he opened the door of your room.

“Probably asking yourself who we’re gonna meet. Well its fucking Dwighty boy, Cause Dwighty fucking fucked it up to keep your damn brother under control”, Negan said grinning.
“And now we’re gonna meet him after one night in paradise”, Negan said chuckling before he went around a corner.
You already saw his doctor leaning against a wall before a locked door.
“Alright and now enjoy the show Sweetheart”, he said chuckling before he let go of your waist and walked in front of the door.
You scoffed as Negan turned once again to you, winking at you before he turned back and drummed his bat against the metallic door.
“Good fucking mornin’, sunshine. Is it just as cozy as you remember? You spent a lot of time on the wrong side of the door after that fucking stupid road trip with the wife and Tina. So let’s talk about now. We went out looking for your little failed project for a few hours, just around the perimeter, tip to taint, as it were, right about the time I had you thrown in here, and when I got back, I realized I was short a wife”, said Negan while he stood before the door.
”Which one?”, you heard Dwights cracking voice ask.
”Sherry. You know anything about that, Dwighty boy? ‘Cause I got to tell you, that is one hell of a coincidence, her leaving just a few short hours after Daryl fucking took off. Oh, and he didn’t force his damn way out. No. Somebody opened the fucking door for him”, continued Negan.
Did she really let Daryl out?
But why should she have done that?
She knew Daryl only from that one time before they got caught as far as you knew.
”It wasnt Sherry”, you heard Dwights weak voice respond.
”Dwighty boy was it you? Did it work the other damn way around? You were supposed to break him. Did he break you? I mean, let’s face it , you’ve got some pretty legitimate grievances. You change your fucking stripes on me, Dwighty? You starting to see things different?”, Negan asked before he pounded the bat again against the door.
“After all this before and after hell, after everything who are you, Dwight?”, asked Negan swinging his bat back on his shoulder.
”I’m Negan”, Dwights voice sounded through the door before Negans hand found its way to the handle and unlocked it.
The door swung open and revealed a bad looking Dwight.
His eyes were surrounded by dark circles and a wound with dried blood was on his forehead.
They had beaten him up and for sure not just for a few minutes and now he sat where your brother had to stay for way too long.
Dwight struggled to get up while you saw how Negan chuckled slightly.
“Daryl isn’t like you. He’s emotional. So he’s either on his way home, or he’s coming back here to try to kill some more of us. Mostly you and me. Either way, we’ll find him”, Negan said while you felt how a shiver ran down your back with his last words sounding through the air.
You knew your brother, you knew how he could be.
But you werent the only one who could handle him, Rick would get everything done, for sure.
At least thats what you hoped.
“So do you think you know where Sherry went?”, Negan said ripping you out of your thoughts:
“Yeah”, responded Dwight sighing.
”Alright then bring her back”, said Negan before he turned to his doctor.
“Stitch him up. Fix what you can fix”, said Negan to the man before Dwight stumbled over to him.
”Oh Miss? We need to get a new bandage for you too in the next days”, said the doctor pointing at your arm.
There was still the wound Arat had given to you when she decided to first shoot you when Rosita was laying on the ground telling she didnt make the bullet.
But Arats bullet just brushed your arm and while Negan made it more than clear to her that she shouldn’t ever dare to shoot you again she shot Olivia.
Negans glance went back to you while he bit his lip while chuckling slightly.
”First thing’s done, Sweetheart now its your damn friends turn”, said Negan as he walked chuckling over to you.
”So you know him good right?”, asked Negan close before you.
”We are both in Rick’s group for a long time now so yes”, you said looking at him before you saw Negan raising his finger chuckling.
Were Sweetheart, you were in the Rick’s group for a long time. Dr. Smartypants is now one of us  and you,..you my dear Sweetheart, you belong to me”, said Negan grinning, his face short before yours while you scoffed shaking your head bewildered.
”I’ll never be one of you”, you growled while Negan chuckled.
”We’ll see Sweetheart, We’ll fucking see”, Negan chuckled before he continued.
”So you know if he has it up here?”, he asked grinning pointing at his head.
”You better ask him yourself”, you growled before Negan slung his arm around you once again.

You walked through the corridors of the sanctuary with Negan chuckling beside you before he opened a squeaking door.
The bright sunlight dazzled you as you walked outside before you saw a familiar face again.
Eugene.
Negan chuckled before he let go of your waist and walked over to Eugene who held a glass with pickles close to his body.
“There he is. Man of the hour! Come on over here, big fella”, Negan said chuckling to Eugene while he walked his arms spreading over to him.
“Don’t be rude, asshole. Say hello”, said Negan chuckling while he looked at Eugene.
“H-H-Hello”, stuttered Eugene looking at Negan before his glance met yours for less than a second.
“You got a name, asshole?”, asked Negan chuckling and you already knew where he wanted to go with that.
“Eugene”, answered he still with a trembling voice.
“Now us. Who are you?”, asked Negan grinning.
Yeah, there was it again.
“I’m Negan”, yelled the crowd of people around Eugene and Negan before he walked closer towards him.
“Well, Eugene I know you remember Lucille. Now, you see this right here? You might have to get real close. That, my friend, is the bullet you made.
Now, under normal circumstances, I’d be showing you that real close over and over again”, Negan told Eugene leaning his body back and chuckling imitating the sound of the bat hitting a head.
“But, Eugene, see, all I really want to know is if you are a smarty-pants. You know things? Answer the question”, asked Negan pointing at him.
“I…I am, indeed, a smarty-pants. I taught myself to cast bullets. I uhm found a machine shop with the necessary stuff. I…I read uh a lot, and, um although my memory is not considered eidetic, I don’t skim and I don’t scrimp. If knowledge is dropped, I do, indeed, pick it up”, said Eugene taking a breath while you watched every move Negan made towards him.
You knew Eugene and besides that you didnt like seeing one of your friends that afraid,
“Oh, so you really are just some asshole?”, asked Negan chuckling.
“N..No. I’m not. I have PhDs in biochemistry, as well as immunology and microbiology, and I’ve completed my doctorate, which makes me a doctor. Prior to the collapse, I was part of a 10-person team at the Human Genome Project, working under Dr. T. Brooks Ellis to weaponize diseases to fight weaponized diseases. Fire with, uh you know uh f-fire”, answered Eugene heavily breathing.
And he was lying again, like he did to the group a long time ago until his lie was exposed.
But he was indeed extremely intelligent and you could understand why he was lying right now.
It was his kind of weapon.
Eugene looked over to a walker held on a few sticks in the cage you saw falling apart just second after your glance wandered over.
“All right, Dr. Smarty-pants. You ought to be able to crack this without breaking a fucking sweat. You see, I have a lot of free labor here at the damn fence, living dead pricks that help keep the riffraff out. Problem is, they don’t keep. They fucking fall apart. Like that poor sack of pile of shit there”, Negan said pointing at the walker in the cage before he continued.
“So, Dr. Smarty-pants, how do we keep them on their feet?”, Negan asked while you saw how Eugene tried get himself under control.
”Goddamn you’re still fucking trembling. Jesus,…well Sweetheart come over here maybe a familiar face will calm his ass down”, said Negan chuckling as he turned around to you.
You looked annoyed at Negan while you crossed your arms before he continued.
”Or maybe you gonna make him even more nervous, who knows”, continued Negan chuckling before he waved you over again.
”Anyways, come over Sweetheart”, he said chuckling before you walked reluctantly over to them.
Negan wouldnt give up anyways.
You looked at Eugene whose glance wandered over to you before he began talking again and you felt Negan sneaking an arm around you. 
Then it blustered out of Eugene, he listed every single thing what was needed and what to do with the molten metal that should protect the walkers.
“God damn! If that ain’t the coolest thing I’ve ever heard in my life! Not only is that practical, it is just badass! Whoa”, Negan said chuckling while Negan let go of you and slung one arm around Eugene’s shoulders.
“Look at you, Dr. Smarty-pants. Did Rick have you doing this kind of fucking valuable stuff for him?”, Negan asked grinning.
”No”, stuttered Eugene as response.
“His loss, our gain. I feel like I need to give you some kind of signing bonus here”, Negan said chuckling to Eugene
“Uh, w-well, I was… I was gifted these pickles.”, said Eugene holding up the glass
“Ahh. No. As a token of my gratitude, I’m gonna send you over a few of my wives to your apartment tonight, show you a good time…Now, I don’t think I have to worry about this. But who knows how truly smart you are? No sex.
That is a grave no-no….However, you can have a little dinner, some drinks, share a few laughs….There is nothing like beautiful women that smell good to make you feel human again”, said Negan chuckling while he got his arm back around you.
“Did…did you say “wives,” meaning plural?”, asked Eugene bewildered.
“Hell yes, I did”, said Negan chuckling.
“Are you-”, asked Eugene looking at you.
”Fuck no she’s none of them”, interrupted Negan smirking Eugene before he continued.
“So what does Dr. Smarty-pants say to his knew bestest friend in the whole wide world? What does he say?”, asked Negan chuckling.
“Thank you. Fully, completely sincerely, seriously thank you”, said Eugene his voice still trembling.
“Okay. Why don’t you go have some fun?”, asked Negan before a blond woman took Eugene and walked inside with him, followed by the other men 
before Negan turned fully to you.
”See Sweetheart, I was fucking good to him”, Negan said placing his arms his other arm also chuckling around your waist.
”Great”, you said with a sarcasting undertone while he looked smirking at you before you heard your stomach rumbling.
”Looks like someones damn hungry”, said Negan chuckling to you.
”Well, lets get you some food”, Negan continued before he walked inside with you.

Before you laid a hall, filled with people and stalls with food.
“I’d love to stay here with you, but I gotta manage some shit. You can have fucking everything you want, just say they should write it on my name”, Negan said before winking at you.
You looked at the crowd before you began walking around.
There wasn’t just food or medicine, there was even a damn barber.
You felt the glances of some of the people on your body while you made your way through the crowd.
After some time you felt your stomach rumbling again and finally stopped before a grill.
“Could I have some of that?”, you asked pointing at the grilled food. “Number?”, asked the man behind it, while you felt his glance already goring through you.
“I dont have a number… but Negan told me to let it write on his name”, you said swallowing.
“Are you fucking with me?”, asked the man growling
“No I…Alright I better go now”, you said knowing that this man wasn’t just a savior he was probably pretty argumentative and you really needed no stress here.
“Wait, wait you can’t just get your lying ass out of here”, he growled glaring at you.
“Alright I don’t wanna start a fight here but I’m not lying. He told me to get it on his Name. You can ask him if you want to”, you said calm but tensed looking at the man.
“You know what were doing with people like you here? We beat the holy hell out of them”, the man said suddenly coming closer while you felt your heartbeat increasing.
He was giant and you knew that if it would come hard on hard your chances would be pretty low, even when you knew how to fight.
Before you could do anything else the man began cornering you against the wall while you still tried to find a way to flee.
“You’re so gonna regret fucking with me”, he growled while you saw how he clenched his jaw and his glare intensifying.
”We don’t have to let that escalate-”, you tried to say as calm as he could but he interrupted you.
”Shut up”, he growled coming even closer.
You balled your hands trying to hold yourself back until you found no single way out anymore.
There was no one helping you and you didnt expect that someone ever would.
Your hands tightened and you felt adrenaline rushing through you body as your fist hit his face making him that shocked that he stumbled back that you could flee out of his grip while you heard the man curse.
You tried to get out of the corner but felt a strong grip at your wrist that pulled you back.
You felt him pulling you at the shoulders pressing you against the wall while you felt his heavy breath already on your skin.
”Goddamn Bitch”, he spatted as you felt how his hand went from your shoulders to your throat while you tried to kick him away with your legs.
“You’re just making it worse”, hissed the man while you kept kicking before you felt his hands tightening around your throat while you desperately gasped for air until the moment the grip of the man suddenly loosened up.

You saw the man sinking blood overflowed down to his knees before you saw Negans bat crushing down on him again.
You gasped for air and felt your heart pumping heavily against your chest while you saw Negan panting and hitting down on the man over and over again that had just seconds before threatened you. 
The head of the man turned into a bashed mix of blood and skull before Negan stopped and you finally got a bit of your breath back.
Negan let the blood covered Lucille fall next to the still twitching man before he walked over to you.
“Holy fuck are you okay?”, he asked heavily breathing while his glance went concerned up and down on you while you could finally breathe a bit slower again.
“Yeah”, you nodded still trembling and trying to catch your breath before Negan turned from you to the silent crowd.
“If any of you dare to do some shit like that I swear you’re gonna end up the fucking same like that dead asshole”, Negan yelled before he turned to one of the people.
“Get his sick ass out of here”, he growled more calm while he stroke over his jaw.
You looked at your, through the punch, red swollen knuckles before you looked up to Negan.
“Punched him?”, he asked while you nodded and a small grin flashed over his lips before he lead you outside the hall.
On the hallway Negan suddenly turned you around to him and began examining your throat with his glance.
His hand went up your head to turn it gently to the side to get a better look even though he was gentle you jolted slightly up.
“Its alright”, you heard Negan say quietly while he kept looking at your throat.
“No marks..”, he muttered before he let go of you but kept looking at you and his hand found his way to your arm where he stroke slightly over it still looking at you.
“You should have screamed for me”, he said swallowing and looking concerned at you while you noticed that he was slightly trembling.
Negan, the cruel Negan, seemed to tremble because of you even when you couldnt really believe it and tried to tell yourself that it was probably just through the adrenaline rush he had through bashing that mans head.
“I’m not gonna ever let that happen again, okay? Trust me at least about that”, he said still looking at you while you slowly nodded.
“Okay. then lets get you away from here”, he said before he began walking.

You walked to the apartment but instead of your room, you stood before Negans.
He opened the door leading you in while you looked at him with an asking glance
“You’re gonna stay here Sweetheart”, Negan said while placing the bat next to the couch.
“I’m what?”, you asked looking bewildered at him. 
“You heard fucking right, you’re gonna stay here with me at least for the next nights”, he said looking at you. 
“Why?”, you asked looking at him. 
“'Cause when your sweet ass is here theres no fucking prick who could attack you again”, he said raising his brows. 
“Why would someone come into my room to attack me? Anyways…you’re also always locking the room don’t you?”, you asked looking at him. 
“Listen I don’t fucking care if I’m locking it or not, you my dear Sweetheart are staying here”, he said looking at you while you scoffed as response.
You weren’t that much into in sharing a room with him.
Your own room here was when you were alone at least a little bit of a save haven for you and if you’d stay here, you knew that you would lose that.
“You’re mostly together with me over the day anyways. So whats the damn problem?”, he asked huffing.
“Well maybe because I liked that little tiny bit of privacy you’ve left me”, you said crossing your arms tighter before your chest.
“I also have no sleeping stuff over here”, you said knowing that this argument was probably pretty weak but you took everything that came to your mind.
“Thats no problem Sweetheart I got something here”, he said before a small grin created on his lips.
And that grin already told that it wouldnt be some normal sleeping shirt.
Negan went over to his wardrobe and pulled a lingerie out he proudly presented.
Goddamn that man was unbelievable. 
“I’m not gonna wear that”, you said while you huffed annoyed. 
“That would be a damn shame ‘cause I’m convinced that you’d look fucking mind blowing in it”, said Negan walking chuckling towards you. 
“I don’t care, I’m not gonna wear that”, you repeated yourself. 
“Well so you wanna sleep naked? I mean I personally would fucking love it-”, he said chuckling. 
“No I’m not. Don’t you have anything else here?”, you asked looking at him. 
“No got nothing… but you can have a damn shirt of mine” He asked looking smirking at you while you just huffed.
And you saw in his face that he clearly liked the idea of you wearing something of his.
“Alright”, you said sighing.
His shirt wasn’t the best option but better than wearing a goddamn lingerie. 
Negan went chuckling once again over to his wardrobe and pulled one of his white shirts out to hand it over to you. 
You took the shirt huffing while he grinned at you before you went over to the bathroom, closing the door behind you and lockig it. 
You needed no unwelcome guest. 

While you saw the dusk already breaking through the window in the bathroom you pulled the big shirt over your underwear and tried to use the small alone time you had right now.
Just in that moment you realized that Negan had actually saved you, you didnt like to admit it but he did.
He did save you through beating someone to death.
He was probably beating people often but he showed his protectiveness over you again and you got more and more problems getting his concerned side along to the cruel side you had seen so often.
But the worse problem you had right now was the weird feeling in your stomach that created when you got to see another side of him and also right now while you thought about it.
There was nothing right about that but thanks to god there was still enough about him that extremely repulsed you and gave you the feeling that you hadn’t gone completely insane. 
You tried to get rid of the rest of your thoughts before you breathed out and unlocked the door to open it.
Negan sat already in the bed, some notebook in his hands while he looked smirking at you.
“Sweetheart I gotta say that doesnt only look cute it also looks damn hot, but what have I fucking expected”, he said winking at you while you walked huffing over to the bed.
You looked over to Negan who lifted the blanket up before you could.
“Come into this cozy bed Sweetheart”, he said smirking to you while you got huffing under the blanket, intentionally leaving some space between you and him.
“Oh come on, Sweetheart, you know that I’m not gonna bite you”, Negan said chuckling while he snapped the notebook shut and laid it next to him on the nightstand.
You sunk deeper into the pillows while you heard Negan chuckle.
“Alright Sweetheart, you don’t have to but I can’t guarantee that we’ll stay that far apart from each other the whole night”, said Negan chuckling before you turned to him and said something that you had never thought you’d say to him but you knew that you had to when you didnt want to feel like a complete asshole.
“I uhm, I just wanted to thank you for what you did in the hall. Thank you, really”, you said looking swallowing at him while you saw a mix between a smile and a smirk create on his lips.
“Anytime Sweetheart”, he said looking at you before you nodded slowly, turned a bit away from him and could to your pleasure doze off shortly after that.

The bright sunlight shining through the windows woke you up.
You slowly opened your eyes realizing that you were in Negans bed and not yours as you saw him laying close to you that close that you even felt his breath on your skin.
You looked at him. His chest went slowly up and down, his face relaxed while a few detached hair strands hung in his face.
It wasnt the first time you saw him that peaceful while he slept but every time it surprised you anew.
And whatever it was seeing him like that, the feeling you had cursed about just yesterday in the bathroom created in you again, while you immediately tried everything to get rid of it.
You didnt want him to make you feel that way.
“Good morning Sweetheart”, Negan said while he opened his eyes looking directly smirking at you.
“What a damn blessed man I am to wake up next to you”, he said chuckling before he sat up and looked at you.
“Alright Sweetheart, I’d love to spend more time with you in my goddamn bed but you gotta get yourself ready so we can get your wound checked up again”, he said pointing at your arm.
You nodded before you made your way to the bathroom, got ready and walked with Negan through the hallways.

Minutes later you found yourself before the doctors office.
Negan took Lucille from his shoulder and knocked the bat again the door.
“Doc, got some job for you”, Negan said but got no response.
“I’m coming in”, Negan said before he opened the door but revealed an empty office.
“Well looks like you gotta put up with me”, said Negan smirking while you walked inside.
You leaned against the table while you watched how Negan took some bandages and the salve the doctor had put on your arm the first time and walked back to you.
He began to get the old bandage of your arm before he began treating your wound while his glance went smirking over and over to your face again.
He got to the end of the bandage and looked for a moment at it.
“Cant leave it like that”, he mumbled before he walked over to the desk and began rummaging in it before he hold a tape triumphically up.
“Got it”, he said chuckling before he looked back to the desks drawer to close it as he went quiet for a second.
“What the heck is that shit”, he asked suddenly holding a small piece of paper up.
“Looks like we found the prick that let your brother out.”

You walked into the hall with Negans arm, as always, wrapped around you while you knew that this wouldn’t end good for the Doctor and you didnt need the burning fireplace for it.
Before you stood the crowd around Dr. Carson who was held by two of Negans men while he looked already like was already completely done with everything.
Which was pretty understandable through the burning iron you saw in the fireplace.
”Alright Sweetheart, its gonna get pretty messy here so in case you don’t wanna watch, I’m not making you. You just gotta close your pretty eyes”, Negan said chuckling to you before he went over to Dr. Carson but still caught you glaring at him.
You looked into the crowd and saw Negans wives stand there, as always in their uniform like black dresses, before something else caught your attention.
Eugene.
He was standing on one of the platforms, next to him stood the woman you had already seen a few times.
You looked back to Negan who stood chuckling before Dr. Carson.
“You are gonna want to pay close attention to this”, said Negan as he turned around while pointing at Eugene with his bat before you suddenly saw how Negan hit Lucille against the Doctor who therefore stumbled and choked
“No, no, no, no! Why? I didn’t do anything”, he whimpered heavily breathing while you looked shocked at the scene before you.
“I found this little souvenir tucked away in your desk”, said Negan holding up the note he had found not long ago.
“I-I-I don’t know what that is”, whimpered Carson before Negan hit his bat once again against him while you jolted slightly gasping up.
“You my dear friend, you left the door open and let my puppy out. You fucking knew Sherry hated Daryl being here, so you let him out for her, be the damn hero. Then you could move in. That is some weaselly shit right there”, growled Negan at the strongly trembling man.
“But I didn’t. She’s the one who ran-”, panted the man but couldnt finish.
“Oh, she ran? You know why she ran? Because she knew I would blame her, which I did. But, see, Sherry told Dwighty boy the whole story right before she was torn apart. A super hot girl, horrifically killed because of your greedy, delusional, and tiny little prick”, hissed Negan before he got the iron while you felt a shiver running down your back only by it’s sight.
“It’s not true”, gasped the man.
“Dwight?”, asked Negan turning to Dwight who stood at the furnace. Your glance landed on him while he just stared cold into the fire.
“He’s lying about it”, Dwight just said while he kept staring into the flames.
“I would never do that”, whimpered Dr. Carson.
“Why? Why? Why would he do that? Why would he intentionally try to hurt you? Sherry’s gone. And if he’s lying and she’s out there, We will find her. And then I will burn the other side of his fucking face off until he dies. So, what was he gonna get out of this?..No I know my Dwighty boy. See, all he needed was one more night in the hole, get his head screwed on straight. It worked before, and it worked now. Ain’t that right, Dwight?” asked Negan turning once again to him.
“Oh, yeah”, responded Dwight still staring into the flames.
You didnt really believe Dwight.
Doesnt matter what he was saying.
Something about his behavior, his glance made you feel like Negan trusted into the wrong opinion right now.
And that would have consequences for Dr. Carson.
“Oh, please. No, please. Oh, Jesus, don’t burn me. Please!  No. No, no, please!“, gasped the man.
“Now you know I hate this shit. Just tell me you did it and that you’re sorry, and I don’t have to do this”, Negan said holding the iron in his hands.
“Yes…Yes, I did it… all of it. Sorry. I’m so sorry. Please I’m sorry”, whimpered the doctor.
“That’s all you had to say. That is all you had to fucking say”, you heard Negan say and saw him looking at Eugene before you thought that you couldnt believe your eyes.
Negan grabbed Dr. Carson by the shoulders and threw him with his head first into the furance.
You jolted up, feeling how your breath got heavier while you stared with widened eyes at the still screaming man Negan held into the flames before he let go of the him and walked over to Dwight.
You tried to get your shocked glance from the hanging out body half while you still couldn’t really believe what just happened.
You knew that you shouldn’t be surprised about Negans cruelty anymore after all what happened, but still you were. 
“Good thing we got a spare Dr. Carson.”, said Negan while he pointed up to the shocked looking Eugene as you asked yourself if Negan knew that Eugene was no healer before you heard Negans voice again.
”Sherry got nothing ever on her”, Negan said pointing at you before he continued.
”But from the wives she was one of my favorites. I’m sorry for you Dwighty”, Negan said looking at Dwight.
“I’m not”, said Dwight with a cold expression on his face.
“Whoo. Fucking ice-cold. I fucking love it”, said Negan chuckling before he let go of Dwight and walked over to you while you still had a mix between glare and bewildered glance in your eyes.
“I think we gotta check a view things before we’re continuing with your friend” said Negan chuckling before he lead you outside with him while you still the pictures of the burning man in your head and all you could think of that was that you hoped that they would never find Daryl, so that he would never have to meet a similar destiny.
You spent the next hour or even hours by Negans side while he was walking through the sanctuary, checking if everything was alright and if there were any News about your vanished brother.
But every time the men and women negated and with that you had at least little bit you could be released about before you made your way to Eugene’s apartment.

Negan smirked at you before he knocked with his bat against the door that slowly opened.
“May we come in?”, asked Negan grinning the slight puzzled Eugene before you before he walked in with you.
“So, how are you liking it here? Are we doing right by Dr. Smarty-pants? You know, Frankie and Tanya had nothing but good things to say about you”, Negan said to the still puzzled looking Eugene.
“It’s all right. I get it. I know how hard it can be to accept change, to get on the right team. My Sweetheart over here? She’s still not into all of this but you know my friend, I’m not gonna give her up”, Negan said chuckling while pointing at you.
“Well but back to you Dr. Smarty-Pants… I need you to understand something. I do not make this invitation to everyone and I sure as shit do not make it lightly”, said Negan while you saw how Eugene began to panic again and you tried to search for his glance.
“Hey. Hey! You do not need to be scared anymore. You don’t need to be fucking scared. You just have to answer me one question. And it’s a big one.
Who are-”
“I’m Negan. I’m utterly, completely, stone-cold Negan. I was Negan before I even met you. I just needed to meet you properly to know. I’m Negan”, you heard Eugene say while you looked swallowing over to him.
Sure, Eugene wasnt the bravest of them all but he couldnt just forget everything Negan did in that night, killed his best friend, and be subject to Negan.
He couldnt actually do that, right?
Was he just playing a game or was he actually making a whole new beginning?
You had no idea and all those questions kept going through your mind while Negan walked with you back to his and now also your room..

part 11


tagging: @imaginesforthepeople @toxic-ink @reylo101 @agespenst @squidgy84 @nobodylastname @xsnak-3x @tolieboy @writteninthestars288 @nice-shoes-nerd @dasani-saraai @thegirlwiththelyrics99 @magical-spit @myrabbitholetoneverland @straightestgay-voice

Your genome, every human’s genome, consists of a unique DNA sequence of A’s, T’s, C’s and G’s that tell your cells how to operate. Thanks to technological advances, scientists are now able to know the sequence of letters that makes up an individual genome relatively quickly and inexpensively.

From the TED-Ed Lesson How to sequence the human genome - Mark J. Kiel

Animation by Marc Christoforidis

nytimes.com
Scientists Talk Privately About Creating a Synthetic Human Genome
The project poses ethical issues about whether humans could be created without parents.
By Andrew Pollack

Scientists are now contemplating the fabrication of a human genome, meaning they would use chemicals to manufacture all the DNA contained in human chromosomes.

The prospect is spurring both intrigue and concern in the life sciences community because it might be possible, such as through cloning, to use a synthetic genome to create human beings without biological parents.

While the project is still in the idea phase, and also involves efforts to improve DNA synthesis in general, it was discussed at a closed-door meeting on Tuesday at Harvard Medical School in Boston. The nearly 150 attendees were told not to contact the news media or to post on Twitter during the meeting.

Organizers said the project could have a big scientific payoff and would be a follow-up to the original Human Genome Project, which was aimed at reading the sequence of the three billion chemical letters in the DNA blueprint of human life. The new project, by contrast, would involve not reading, but rather writing the human genome — synthesizing all three billion units from chemicals.

But such an attempt would raise numerous ethical issues. Could scientists create humans with certain kinds of traits, perhaps people born and bred to be soldiers? Or might it be possible to make copies of specific people?

4

‘Good evening, everyone. I am the Detective Prince, Naoto Shirogane.

Welcome to “Experiment of the Century: The Genome Project.” I will be experimentor and experimentee both, in a forbidden yet wonderful bodily alteration process. You shall witness my departure into a new realm … The moment of a new birth!

From the chosen day forth, I shall walk a completely different path in life … ! And I will share this glorious occasion, this memorable day, with all of you!

Do stay tuned!’

That “dude with a lot of money” kept insisting I was a “surfer model” and I told him I was a scientist working on the human genome project (I know it’s completed or whatever) and then he asked if I would be interested in flying with him to New York and I was like what? no. And then he asked my opinion of him and I was like “dude straight up you come across super arrogant. You keep saying you don’t care about money WHILE TALKING ABOUT MONEY it’s not attractive.” and he was like “oh no! But I don’t care about money!” And my sister got him to but the group multiple rounds of drinks so at least they got something out of that walking shit show.

10

Hey! 

Happy National DNA Day! Did you know the Human Genome Project was completed on April 23, 2003? 

To celebrate, here’s a short comic to teach you how to spot left handed and right handed DNA. Once you know how you can shame media outlets who didn’t bother to check their stock photos educate other people about DNA chirality!

TMNT Flashback . . .

“April, you’d better head to the lair where it’s safe.”

“We’ll go check out the Worldwide Genome Project.”

“But first, we gotta take down the East Side High Panthers.”

“According to that poster, they’ve got it comin’… ”

” … “


Click here for more TMNT Flashbacks … ;)

So I just realized what Oikawa meant in 02 episode 46 when he said he was working on genome analysis. Both he and Mr. Ichijouji are geneticists, likely working on the Human Genome Project.

First of all, this means they’re REALLY GODDAMN SMART. People who work at genome analysis, especially important international government-funded undertakings like the Human Genome Project, are top tier research scientists, with all the credentials to show for it, meaning both Oikawa and Mr. Ichijouji have PhDs in the relevant fields of study. I feel a need to apologize to Mr. Ichijouji for discrediting his intelligence compared to his hyper-intelligent sons, because it is clear to me now that he has worked his brain extra hours to get to be the chief of a team of fucking geneticists.

Second of all: Arukenimon and Mummymon’s creation suddenly makes SO MUCH SENSE. It was right there in front of me for years and I just didn’t get it. But seriously. Question: How do you digitize a human being’s DNA? Answer: DNA SEQUENCING. The Human Genome Project already had a rough draft around when Arukenimon and Mummymon were created, and Oikawa was already working on creating them before Hiroki died (he says as much in episode 47). There is absolutely nothing stopping him from sequencing his own DNA and using that and some input from the spirit of a brilliant psychopathic Digimon lodged in his head to create artificial Human/Digimon life. Still very much steeped in the realms of fantasy science, but dude, this is Digimon we’re talking about, it’s already fantasy science.

Basically I’m dumb and Oikawa and Mr. Ichijouji are really smart and I understand so much now.

If you’re still reading this thank you very much, have a photo of Oikawa from October 1999 looking grumpy in a labcoat and you probably deserve a doodle or something you’re awesome.

Sorry followers I am interrupting my usual blogging because something has really bothered me today.

My fellow deValier fans. A user known as The Fox Familiar has started reviewing George’s stories. This user is known on FF.net for flaming and abusing writers. Please read their profile page on fanfiction.net to see the homophobic, misogynistic hate speech they come up with. If you plan on replying to them, do not go to the tumblr user link provided as it is not the actual person. I ask that you help get this user blocked. This user is simply a bully. 

They actually asked George in the review…“Second, why is Feliciano gay? If this is some sort of AU where all forms of ‘love’ are accepted, I wouldn’t expect the Italian love of tradition to go out the damn window. If Italian fascism is anything to go by, it’s that they look down harshly on homosexuality, and this, too, would apply to Germany”.

It’s as if they think that because homosexuality was hated at the time, there would be no homosexuals. Because obviously being gay is a modern creation and a choice (sarcasm). Also notice the quotation marks above love. 

These are some of the other things that have been said on their page.

“And yet since the success of the Human Genome Project a decade ago, no one has yet spelled the gay gene? I wanna see some As, Gs, Ts, and Cs, people!”

“I think you honestly took my profile WAY too seriously, sweetheart. Had you read my profile more carefully, you’d see that I’d advocate the murder of those who are detrimental to society; those who think that their lives are the centre of the universe. Some people do live worthless lives, and it should be society’s role to get rid of them, not keep them.”

“I should also mention that homosexuals make up the vast majority of paedophiles. So it’s not surprising to me that you like underage porn. In reality, you are a man, born a man, and forever will be a man, and no surgery and political correctness can change that. By saying you are a woman, you are committing the act of MANIPULATION, and hiding what you really are. That is immoral, wouldn’t you agree?”

“You support a lifestyle, and even engage in, a lifestyle that is unnatural and unhealthy. You’re a lesbian - so what? I don’t care. I know that you’ll probably get bacterial vaginosis or HPV, and probably your girlfriend will leave you. Or, you’ll engage in drug activity and alcohol abuse”.

“So, you constantly tell me that I am such a bigot, that you can’t believe people like me exist. Yet you state that watersports/scat is strange, yet go on to tell me that other sexual deviances are A-OK. You try to tell me that I am unintelligent; you try to tell me that homosexuality et al is perfectly fine and demand that I watch videos of anal sex. Well dear, I have a thing called a book on physiology, and it shows that the anus was in no way designed for constant penetration. Since you oh-so-forget this fact, I quite correctly assume that you are an idiot”.

“Then you assume that I am religious. Of course you’d attack Christianity, which is responsible for the DON’T HATE ME FOR WHAT I AM values that you use here (oh, doesn’t that hurt?) but you’d NEVER attack Islam or Judaism; the former because of racism and the latter for anti-Semitism. Besides, the Bible shouldn’t be taken literally, but I do know that Christianity is responsible for the rise of science and why we are such moralistic individuals that don’t kill each other on a daily basis like savages. Of course you’d attack Christianity and religion, because it RIGHTFULLY condemns your unhealthy behaviour.”

Please report this person  through twitter to FF.NET. They are known to tell writers to go kill themselves. No matter what your views on slash parings or fanfiction are. Nobody should be spoken to like that.

And now my usual blogging will resume. 

me: i’m gonna get so much done on my genomics project today!

me half an hour later only partway through the annotation of 1 out of 4 genomes: i’m gonna get one thing done today! 

Intellectuals in their self-flattering wish-fulfillment say that knowledge is power, but the truth is that knowledge further empowers only those who have or can acquire the power to use it.
—  R.C. Lewontin

anonymous asked:

After finally saying enough is enough, I broke away from Christianity and decided to be a pagan. I've been interested in Egyptian dieties since I was a girl (but of course was punished for it). I haven't read a single book. I don't know what an altar is or how to make one in my home. I don't know how to "talk" to dieties or how to distinguish them. I only know of maybe 2 Egyptian dieties. :/ Oh and I'm white. What do you recommend for me, the blind beginner?

Hi anon.

Well, there are a lot of things to address in this ask, so let’s get started.

Race

The race debate is one of those things that can get pretty hot and heavy when it comes to ancient Egypt. I am not going to get into it as much as I could, but I will leave you with this link so that you can get an idea as to what the controversy is about. A lot of people, mostly due to the ideological train of thought of Afrocentrism, have come to believe that Kemeticism is barred to anyone who is not of African descent. However, something that many people seem to forget is that the genome mapping project has begun and thus far, results are showing that, genetically, the ancient Egyptians are no different from those that are found there today. For anyone interested in the archaeogenetics of ancient Egypt, this should give you a general picture.

The ancient Egyptian religion was an open religion in antiquity. They readily and willingly accepted converts from other cultures and even added gods from other cultures it their own. As long as the convert was willing to live in ma’at, they were pretty much all set. Another sticking point is that the religion died out. It is not the national religion of the culturally Egyptian. In fact, modern-day Egyptians tend to look down upon their ancient heritage, in all honesty. The country is very devout, but to a single deity as opposed to the many of ancient Egypt. While some things have passed down to the modern populace, these are mostly superstitions carried out as opposed to any form of devotion to the ancient gods.

Kemeticism

When it comes to learning about Kemeticism, there are a couple of different avenues that can be explored here. I recommend taking a look at my resource list as well as this information page by Devo. Devo’s page give a broad overview of what Kemeticism is about. She also provides forums, books, temples, and other information for your perusal. My resource list provides a list of books (historically based books for the most part) as well as a list of bloggers and webpages to peruse for information.

When it comes right down to it, when you first push off the yoke of a previous religious tradition, you’re going to have a lot of questions. And this is going to get scary. And this is going to be something where you end up feeling very out of your depth for a lot of the start. Just know that everyone felt that way. Everyone walked into a new religion, whether it is Kemeticism, Druidry, Hellenismos, etc. We all have no idea what we’re doing, what books to read, or where to even begin. I strongly recommend following the Kemetic and Kemeticism tags so that you can read others’ experiences, questions, worries, etc. And of course, every single one of us has our asks, which are open to anonymous questions.

Altars

Altars are usually one of the first place that new pagans go to because it’s something physical that they can do. And of course, I highly recommend creating one. I don’t recommend creating it in an effort to a single deity. Deities, in my experience, tend to make the choice as to who they will reach out to (or not) and there’s no point in forcing the issue. They may not respond to your entreaties and that may make you feel bad about what you’re doing. Even if you decide to ignore my advice, don’t feel bad if you don’t get any responses from the gods. They’re fickle and do what they want. You just keep doing whatever you feel like you need to do.

For a general altar, I recommend finding a flat surface that won’t be disturbed by others. This can be a nightstand or a bureau or a corner of a desk or a bookcase shelf – whatever you have handy. I recommend placing a small candle (either flamless or a tealight) in the center. This can be your focal point. As to what else you place on it, it’s really up to you. You can place flowers, pretty stones, jars, divination tools, or whatever you really want on it. It’s up to you. If you live in a place where incense is out, then you can always get one of those scented oil packs from the dollar store or one of those scented room cleaner things made by Glade or something to use instead of incense.

I recommend having a general altar to get you into a groove. This groove will help you to begin communicating, praying, or at least parsing out what you want to do when it comes to this religion stuff. By not having an altar dedicated to any deity, then you can reach out to all of the deities you feel like reaching out to, or not. I just recommend, honestly, at the start to get in the habit of spending, like, five minutes of your day in front of that space to build you up. If a deity shows up, then you can start adding things that you feel that deity would like to make it their altar. And if you’ve been doing the plunking down in front of the altar thing for five minutes a day, it won’t seem so strange when you start providing offerings to deities.

Talking/Praying

When it comes to talking to the gods, I honestly recommend just sitting down in front of whatever surface area will be your general altar and just start blabbering away. I don’t think any formality is really necessary. You can introduce yourself in a sort of general way: “Hi, my name is _______ and I’m interested in Kemeticism.” And then take it from there.

I tend to associate these conversations with conversations you would have with people you just meet. You’d want to introduce yourself. You’d want to tell them about who you are, why you are, and what’s going on in your life. From there, you can move on to talking about why Kemeticism interests you and why you think the netjeru (the gods) are the way to go.

Distinguishing

Telling one netjer from the other can be difficult, especially for people who are new to all of this. There’s no discernment in the beginning. Every experience can be something new and exciting and possibly a sign from the gods. That’s not necessarily the case, but I think that new people look to things in that way because they want to feel a deep, personal, and powerful connection with the gods as quickly as possible. That’s not always going to be the case. As I said above, the gods are fickle beings and do whatever the heck they want when it comes to “picking up” new devotees.

Devo wrote this post about discernment and I strongly recommend you read it.

In order to distinguish once Kemetic god from another, I strongly recommend bookmarking the Henadology page for future reference. I also recommend finding either an eBook copy or a cheap copy of the following two books: The Gods and Goddesses of Ancient Egypt by Richard H Wilkinson and Egyptian Mythology by Geraldine Pinch. These two books, and that webpage, can give you clues that may help you to figure out what deity, if any, is reaching out to you. There are certain aspects to iconography and the myths that can provide those clues.

Advice

Take it easy.

Read all the historical resources you can.

Take it slow.

Have fun.

Go with the flow.

Ignore everything about Horus/Isis/Osiris being the blueprint for the Christian trinity.

Ask all of the questions.

E-stalk all the blogs.

Do not read anything by Wallis Budge.

Ignore everything you see about Atlantis and ancient Egypt.

Don’t let anyone tell you that you’re doing it wrong.

Be good to the community.

Don’t get scared off by the Afrocentrism in the tags.

Ignore everything about aliens in the tags.

Further Reading

My guides & 101s.

Devo’s starter guide.

Devo’s offering guide.

Kemetic Round Table.*

* The Kemetic Round Table (KRT) is a blogging project aimed at providing practical, useful information for modern Kemetic religious practitioners.