fluorescense

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Removing fluorescein from the organic phase. During the reaction an amide of fluorescein was made what doesn’t have a color and it does not fluoresces under these conditions. So hopefully, the transparency of the upper layer means something good.

The bottom layer is a dilute (1M) sodium hydroxide solution what reacts with the fluorescein to form the highly fluorescent fluoriescein sodium salt what produces this intense green color in the aqueous phase.

Interesting fact: the acid form of the fluorescein does not emit the characteristic intense green color under UV light, it only emits a yellowish-orange color in high concentrations.

8

Made some ace-themed bracelets and necklaces. Or as I’ve been calling them: brACElets and necklACEes.

lolpuns sorrynotsorry

Meaningful colours to those in the know; just some pretty jewellery to those who aren’t. Or wear your ace ring on a cord around your neck instead of on your fingers if you’re like me and don’t much care for wearing rings and/or work in an industry where doing so is dangerous.

The longer bracelets also work well as anklets. And yes, these do indeed fluoresce under ultraviolet light. Was difficult to photograph the glow, but with a little bit of editing I got it pretty close to how they look in meatspace.

They’re braided out of nylon cord (8-strand braid), with silver-tone end caps and lobster-claw clasps, and the necklaces have 5cm chain extensions. The clasps and chains were labelled nickel-free but I can’t recall if the end caps were, so proceed at your own risk and all that jazz. End cap diameter is ~5mm, so they might fit through the holes in some of those build-your-own-bracelet charms that are popular right now (maybe throw a cake charm on there?), though the jump-ring will probably need to be removed first since it’s a bit wider than the end caps.

Bracelet measurements and amounts:
19.70cm (7.75") x2
20.30cm (8.00") x2
21.60 cm (8.50") x1
22.90cm (9.00") x1
23.50cm (9.25") x1

Necklace measurements and amounts:
35cm (13.75") x1
40cm (15.75") x1 SOLD
45cm (17.75″) x1
50cm (19.75") x1

Bracelets are 45 NOK each, and necklaces are 90 NOK (converter over here to check what it is in your local currency), plus whatever shipping comes to, which probably won’t be much because these are small and light. Will ship all over the world. (Obviously this means anyone who wants one needs to be okay with providing a mailing address.)

Also accepting art trades of equivalent value in lieu of money.

PM me if some of these strike your fancy. First to call dibs, gets.

youtube

i wouldn’t mind, perhaps, spending a little time with you.

directed by Stéphane Sednaoui

4

Column chromatography of a small part of the previously done reaction. The test tubes and the column looks like I’ve been purifying fluorescein if it is viewed under UV light.

With this method I am able to separate the fluorescent compound from the other compounds what are present in the reaction mixture and it will be possible to find out that what have formed in the flask yesterday. The good point is, that it is not a black gunk(:

Something strange happened here. The compound what I started from fluoresces with a deep green color and the compound what I am making fluoresces under UV light with a deep blue color and not this intense yellowish-green color. Conclusion: a side reaction happened and according to the TLC something went really wrong.

Let’s find out that what was produced from this reaction… I hope that nobody added some fluorescein to my reaction while I was not in the lab…

The 850.-th post on the blog is from things what never-ever gets boring: fun with fluorescent dyes!

The red color is emitted by a fairly common dye: Rhodamine B. The green color is a bit more interesting, it is emitted by merbromin, an organomercury chemical: 

Merbromin is one of the best antiseptics and it is still used in several countries, but because of its mercury content, it is no longer sold in the United States, Germany, or France. When used as a topical antiseptic, it stains the skin bright red and it is quite hard to remove. Luckily it is only used as a 2% solution, or more dilute, and since it is not readily absorbed by the skin, it is perfectly safe to use.

If you would like to purchase this picture, please visit: Society6 -FREE WORLDWIDE SHIPPING thru June 8, 2014!

2

Fluorescein sodium salt and a little Rhodamine 6G in a glass tank. This is not interesting at all, the only “special” about this is that I got a flashlight for a few day from a friend and I tried it out with this. 

The flashlight is a cheap Yongnuo YN-460 (~50 USD on eBay) what also emits some UV light what produces this nice green/red fluorescence with the fluorescent dyes.

A solution of ditosyl erythritol under UV light. As the reaction completes the green color should fade, since it is caused by the not yet reacted ditosyl compound. 

It could be pretty useful if one of the compounds what you work with fluoresces under UV light, since it could be detected in no time with a simple UV lamp, that is is present if the reaction or not.

There was a reaction what produced an intense fluorescent side product what I purified by column chromatography a week ago. After the purification I ended up with this oil what looks like this under UV light. It is simply fascinating, it looks adorable, or at least I think this about this small flask with the few mg of purified compound in it.

The best pictures from the blog, what are tagged as my portfolio, including this picture, could be purchased at Society6 as a high quality great looking print: http://society6.com/labphoto.

Two pieces of fluorite under UV light.  

Fluorite (CaF2) is naturally occurring mineral, it is the far most common and well-known fluorine containing mineral found on earth. When pure, it is completely colorless and transmits light from 200 nm what means it lets through UVA, UVB and some of the UVC photons.

But when it is contaminated with other elements, usually with yttrium, cerium, iron, sodium, barium, aluminium, ect. it could change the color of the mineal and could also cause a fluorescence under UV light, just as in this case. Originally the fluorite on the right had a green color, but it emits strong blue light under UVB.

I know, that this is not strictly chemistry, but is there anyone who would like to see some mineral related posts on the blog in the future?

4

Not from the lab,  but something interesting (or at least it’s interesting for me). 

I tried, that various photographic lenses how will behave under UV light and got quite interesting results. I borrowed a few lenses from a really good friend and started to do some experiments. Some of the lenses fluorescence under UV light with blue/green/yellow/ect. color. 

Why do they glow under UV? Depending on the composition of the glass, it could easily contain mixtures a few rare earth elements, usually Lanthanum, Cerium and sometimes Thorium (yes, that radioactive thing at the bottom of the periodic table). These elements are added to the glass, since if they are present, the glass has a higher refractive index what means, that better optics could be made from them, with lower chromatic aberration.

What is chromatic aberration? It’s also called achromatism or chromatic distortion, is a type of distortion in which there is a failure of a lens to focus all colors to the same convergence point. It occurs because lenses have different refractive indices for different wavelengths of light. The refractive index decreases with increasing wavelength. An example.

The first pics, from the fisheye lens and many adorable looking picture from the blog could be purchased at Society6, now with free worldwide shipping: http://society6.com/labphoto/prints?show=new&promo=d28ef1

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Lenses under UV light on Behance I tried, that various photographic lenses how will behave under UV light and got quite interesting results. I borrowed a few lenses from a really good friend and started to do some experiments. Some of the lenses fluorescence under UV light with blue/green/yellow/ect. color.  Why do they glow under UV? Depending on the composition of the glass, it could easily contain mixtures a few rare earth elements, usually Lanthanum, Cerium and sometimes Thorium (yes, that radioactive thing at the bottom of the periodic table). These elements are added to the glass, since if they are present, the glass has a higher refractive index what means, that better optics could be made from them, with lower chromatic aberration. An example. What is chromatic aberration? It’s also called achromatism or chromatic distortion, is a type of distortion in which there is a failure of a lens to focus all colors to the same convergence point. It occurs because lenses have different refractive indices for different wavelengths of light. The refractive index decreases with increasing wavelength.
For more, please visit: https://www.behance.net/gallery/18511343/Lenses-under-UV-light