Separation of a highly fluorescent anthranilic acid derivative from the reaction mixture.
The upper organic layer dissolved almost completely my compound from the reaction mixture and could be separated in one step. A good point was that the compound had a really strong fluorescence and if I placed an UV lamp next to the separation funnel it was easily observed that the water phase contained almost none of the title compound.
This white anemone flower is pretty cool in UVIVF. Just between the teal part of the petals and the outer pink there is a darker band of red. In visible light, that appears to be the only part of the petals that isn’t white. Neat!
I love working on fluorescent probes 😍 this is a ligand i synthesized to have a nucleophilic site so it can be attached to a solid support bead. The idea is the bead+ligand will bind in the active site of a special type of luciferase, and pull down protein-protein interactions for that special luciferase.