T4 Ligase is an enzyme that is used to bind two strands of DNA together. The Ligase enzyme creates a new bond between the 3’ end of one DNA strand to the 5’ end of another. Ligation reactions are used in molecular biology to insert genes of interest to plasmids for plant / bacterial transformation and cloning.
Thank you so much to everyone that watched my Periscope live stream today! This is the end result of today’s work, an overnight T4 ligation to combine two pieces of DNA for future experiments!
I will be doing more live streams in the future so please be on the lookout for those. If you have Periscope downloaded on your phone, follow me @dailyplantfacts to get notified when I start a new broadcast!