Listen ya’ll. Trigger has taken too long by now if they wanted to reveal Hannah and Barbara are related to one another. I highly doubt that they are blood-related. I know in Kill La Kill they took the whole series to reveal Ryuko and Satuski being sisters but Ryuko didn’t KNOW that they were related (im pretty sure), whereas in LWA Hannah and Barbara definitely know what they are to each other (even if we don’t).
But they don’t call each other “nee-chan” or “onee-chan” or “imouto”. I fully believe they are just very close, very catty best friends who have been together for as long as they can remember. Probably rich neighbors from childhood who both admire Diana and they bonded over that and a lot of other things to become inseparable.
I mean at this point, if they WERE related, it’d be a stupid move for Trigger to wait this long to reveal it, since they’ve dropped some hints that Hannah and Barbara are a lot closer than just ‘best friends’.
Either way, don’t try to freak me out about it. I won’t ever believe they are related. Let them be gay best friends. Let me have this.