actin filaments

3D organization of actin filament-based stress fibers at the leading edge of a crawling osteosarcoma cell. This cell was imaged using a super-resolution imaging technique called structured illumination microscopy which yields double the resolution of conventional fluorescent imaging techniques and reveals the 3D structure even in this very flat structure. The Z-positions of actin filaments are color-coded using the rainbow (red, orange, yellow, green, blue, purple) with red representing structures closest to the growth surface and purple at 2.5 microns above the surface. Image : Burnette Lab.