Estoy en un punto donde enserio no quiero nada, y no me dan ganas de nada, solo quiero acostarme y quedarme ahí, esperando algo que no va a pasar.

Infectious Bronchitis Virus infection in chickens 

Yo cuando dije que este seria mi año.

— Jenelyn Borres.

Escritor de sueños

Mi estefish

Siempre te voy a querer un poquito más de todo lo que te digo

Estefish

Cute Components of Blood

These prints can be found here :) 

Canine reproductive cytology

We had a practical demonstrating the oestrous cycle in the bitch using vaginal cytology, so here’s a brief summary of some key points. (I also must apologise for the poor quality of the pictures…).

Vaginal cytology can be useful to detect pro-oestrous, oestrous, and dioestrous, which is necessary to facilitate accurate breeding management. Before I introduce cytology, here’s a recap of the oestrous cycle in a dog:

Pro-oestrous is approx. 5-9 days long. Oestrogen is dominant.

Oestrous is approx. 6-12 days long. Ovulation occurs ~2-3 days after the LH surge. The fertilisation window occurs 2 days after ovulation, and lasts ~4 days. Oestrogen is declining, and progesterone is increasing (begins prior to LH surge). 

Dioestrous can be 2-3 months (non-pregnant) or 57 days (pregnant)

Anoestrous lasts around 4 months.

In cytology, the main index that is used is the Superficial Cell Index (SCI) which estimates the percentage of superficial/dead epithelial cells present, compared with intermediate, parabasal, and basal cells. Below are images of each cell type:

Superficial

Intermediate

Parabasal (also with intermediates)

Basal 

Cytological signs of pro-oestrous include copious eythrocytes, some neutrophils, debris, and around 60-80% SCI. Non-epithelial cells and debris decrease leading up to oestrous.

Cytological signs of oestrous include very little debris or non-epithelial cells, and 100% SCI. The thickness of the cell layer increases as oestrous progresses due to cell sloughing. 

Cytological signs of dioestrous (D1) include a rapid decline in SCI by >20% until <10% SCI. Living epithelial cells increase by >10%. This indicates the end of the fertilisation period. D1 is useful in estimating the whelping date if the bitch becomes pregnant.

Reviewing my Vet. anatomy because we had oral recitation back then.

No ignores el esfuerzo de una persona que quiere quedarse contigo, no todo el tiempo a alguien le importas.

—

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Quiero llegar a casa,

escuchar tu día,

y que escuches mi día,

mientras compartimos una taza de café,

y compartir miradas,

mientras deseamos besarnos,

hasta el alma.