Ryan Returns Home from ICU

Ryan Returns Home from ICU

Ryan was discharged — directly from the ICU — and is now at home. We were fortunate to get out when we did because our area is getting quite the progression of precipitation: freezing rain, sleet, ice, and then snow.

Ryan gets his IV antibiotic this morning.

Over the next 14 days I must give him a strong (and somewhat uncommon) antibiotic intravenously. It is for the bacteria, serratia, that…

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Crude Cacterial Consortium Biosurfactants Potent for Bioemulsification of Oils and Petroleum Hydrocarbons

Crude Cacterial Consortium Biosurfactants Potent for Bioemulsification of Oils and Petroleum Hydrocarbons

Jyotsna Kiran Peter1*, Abhishek Kumar Rao1 and Ritu Kumari1

Department of Microbiology and Fermentation Technology (MBFT), Jacob School of Biotechnology and Bioengineering (JSBB), Sam Higginbottom Institute of Agriculture Technology and Sciences (SHIATS), Naini, Allahabad, Uttar Pradesh, India, 211007

*Corresponding author: Jyotsna Kiran Peter1*, jyots.kp@gmail.com

 Abstract

Crude biosurfactants…

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Development and immunity-related #microRNAs of the lepidopteran model host Galleria mellonella

Background: Micro#RNAs (#miRNAs) are small non-coding #RNAs that act as key players in the post-transcriptional regulation of protein synthesis. Although little is known about their role in complex physiological processes such as development and immunity, our knowledge is expanding rapidly thanks to the use of model systems. The larvae of the greater wax moth Galleria mellonella are now established as model hosts for pathogens that infect insects or humans. To build on our previously-reported comprehensive G. mellonella transcriptome, here we describe the identification and analysis of development and immunity-related #miRNAs thus providing valuable additional data to promote the use of this model host for the analysis of complex processes. Results: To screen for #miRNAs that are differentially expressed in G. mellonella (1) during metamorphosis or (2) following infection with the entomopathogenic bacterium Serratia entomophila or (3) with the parasitic fungus Metarhizium anisopliae, we designed a microarray containing more than 2000 insect #miRNA probe sequences. We identified #miRNAs that were significantly expressed in pre-pupae (16), pupae (22) and last-instar larvae infected with M. anisopliae (1) in comparison with untreated last-instar larvae which were used as a reference. We then used our transcriptomic database to identify potential 3[prime] untranslated regions that form #miRNA-#mRNA duplexes by considering both base pair complementarity and minimum free energy hybridization. We confirmed the co-expression of selected #miRNAs (such as miR-71, miR-263a and miR-263b) with their predicted target #mRNAs in last-instar larvae, pre-pupae and pupae by RT-PCR. We also identified #miRNAs that were expressed in response to infection with bacterial or fungal pathogens, and one #miRNA that may act as a candidate mediator of trans-generational immune priming. Conclusions: This is the first study to identify #miRNAs that are predicted to regulate genes expressed during metamorphosis or in response to infection in the lepidopteran model host G. mellonella. http://bit.ly/1tCrfxR #BMC

Cultivo de Serratia marcescens Culture of Serratia marcescens on Flickr.

AIRLIFE te pregunta ¿qué importancia tienen las bacterias? Tienen una gran importancia en la naturaleza, pues están presentes en los ciclos naturales del nitrógeno, del carbono, del fósforo, etc. y pueden transformar sustancias orgánicas en inorgánicas y viceversa. Son también muy importantes en las fermentaciones aprovechadas por la industria y en la producción de antibióticos. Desempeñan un factor importante en la destrucción de plantas y animales muertos.

Analysis of individual and bacterial consortium of broth culture of Pseudomonas aeruginosa, Serratia marscecens and Bacillus subtilis to emulsify oils and hydrocarbons

Analysis of individual and bacterial consortium of broth culture of Pseudomonas aeruginosa, Serratia marscecens and Bacillus subtilis to emulsify oils and hydrocarbons

Jyotsna Kiran Peter1*, Abhishek Kumar Rao1 and Ritu Kumari1

Department of Microbiology and Fermentation Technology (MBFT), Jacob School of Biotechnology and Bioengineering (JSBB), Sam Higginbottom Institute of Agriculture Technology and Sciences (SHIATS), Naini, Allahabad, Uttar Pradesh, India, 211007

*Corresponding author: Jyotsna Kiran Peter1*, jyots.kp@gmail.com

Abstract

Three bacteria namely,…

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