myotubes

I’m back, folks !

Like I said, I got an internship in a lab, I spent two months there doing cell cultures, fluorescence microscopy and a lot of reading. Despite my heart made of stone, I got the urge to cry twice, when I first used the confocal microscope, and when I left it. Actually no, I also almost cried out of frustration when the Leica TCS SP2 bugged out on us and would not work until the end of my internship. Fortunately, we had only a week without a microscope, until we switched to the Olympus FV-1000.

I can’t actually show you any of my work because it’s not published yet. Depending on when it’s actually submitted and when (if) it’s accepted, it might take a while before I can show my beauties. They were C2C12 (muscle) cells, like the ones above, and although they died on me quite a lot, they were a joy to hang around with. 

This was Frontal Cortex, anthropomorphising cells since forever.

See you !

Today huge news, the article I’ve worked a bit for is on the cover of Journal of Cell Biology.

Above, the cover picture : Quick-freeze, deep-etch transmission electron microscopy of myotube plasma membranes shows an abundance of large clathrin lattices (depicted in various pseudocolors) associated with branched actin filaments. Vassilopoulos et al. reveal that these clathrin plaques help to organize skeletal muscle sarcomeres and attach them to the muscle cell membrane.

Vassilopoulos S. et al. (2014) Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J Cell Biol, 205 (3):377