This research estimates the agricultural production Water Footprint (WF) of Chile, assessing the green, blue and grey WF of the main agricultural products for the main productive regions, taking into account climatic and soil differences. The agricultural production WF is currently 9,509 MMm3/year, which is equivalent to 573 m3/person/year. Even though it is lower than the world´s average, its blue component is significantly greater than the world´s agricultural blue WF. The areas where the blue WF is higher present, at the same time, less water availability. Thus, agricultural production in Chile, a semiarid country, is comparatively under greater water stress. In this scenario, public policies are required to incentivise better water management in order to reduce water vulnerability while boosting development. Using estimates of WF in public policy design may help Chile’s agricultural development in a competitive way. Chile´s agricultural transformation between 1950 and 2007 has led to a significant reduction in the country’s agricultural production WF.