biosystems

I’ve been all about goats lately. My mother wants to start raising chickens at her house but the neighborhood is full of cats, not to mention burglars. Chicken burglary is real, people. But goats? The roadrunners might give a goat some attitude but he’d sling it right back, and I’ve heard their not bad as guards. Apparently they’re pretty social though, so you’d need a couple of ‘em, and if you want any milk they have to keep kidding. Dilemmas, dilemmas.

2

I actually put in actual effort today. I went to engineering open houses and I’ve found it guys. I know for sure I want bio systems. I really want to make a difference and be personal about it. Seeing those students talk and the professors, everyone was so close. I absolutely loved it and all the senior design projects that we saw, oh it was sooo intriguing. I can’t help myself I was like a kid in a candy shop. In other news I’m back to training with running, with the better weather I needed it. My aunt wants to do a half marathon with me so I need to get back to lonnggg distances. No more 4milers. I hope everyone had an awesome Tuesday, I’m looking forward to hearing about everyone’s day just saying! Happy Tuesday!

Jesse Jacobs’ pantone panoply Safari Honeymoon has been reviewed by A.V. Club.

“When reading Jesse Jacobs’ Safari Honeymoon the primary feeling is one of total immersion. It’s not just a graphic novel about a newlywed couple taking their honeymoon in a strange, dangerous environment, it’s a sensory experience that engulfs the reader in an intricately detailed biosystem of horrific parasites, alien foliage, and geometric spirits.” — Oliver Sava, A.V. Club

Read the whole review here!

SMRT Sequencing

Next generation sequencing is all the rave in genetics laboratories nowadays, it seems like everybody and their mums’ able to get the sequence of the DNA they’re interested in. DNA sequencing wasn’t always as easy to do as it is now. Sanger sequencing was invented in 1977, a slow, laborious and expensive process that was used for over 25 years. It was only in the mid 90s that next generation sequencing was being developed, and it was only brought into use around 2004.

SMRT (Single Molecule Real Time) sequencing is a highly advanced next gen process, which was developed by Pacific Biosystems. It was first used in a paper published in Science in 2009, by Eid et al.

SMRT sequencing uses DNA polymerase, an enzyme that synthesises a new DNA strand out of individual nucleotides, using an existing single stranded DNA molecule as a template. If you can watch which nucleotides the DNA polymerase incorporates, you will be able to find out the sequence of nucleotides as the DNA polymerase processes along the template DNA.

To visualise which nucleotide is being incorporated, the nucleotides are labelled with a fluorophore. Fluorophores are small molecules that absorb light and re-emit it at a new wavelength. The four different nucleotide bases (G, A, T and C) are each labelled with a fluorophore of a different wavelength, so they can be told apart.

The process itself is an elegant exploitation of the natural workings of DNA polymerase, shown below.

As the DNA polymerase lines up the correct nucleotide base with the complementary base of the template DNA strand, the fluorophore shines in what’s called a fluorescent pulse. When the pyrophosphate group bound to the nucleotide is cleaved off by the DNA polymerase, the pyrophosphate and the attached fluorophore diffuse away, ending the fluorescent pulse. The energy from the breaking of that bond is used to form the phosphodiester bond between the current nucleotide and the previous nucleotide.

The DNA polymerase then translocates the template DNA along to the next base, and waits for the complementary nucleotide to diffuse into its active site, which will then provide the next fluorescent pulse in the sequence.

It’s not quite as simple as that though. DNA polymerases don’t synthesise DNA at a constant rate, they start and stop. This means to get the sequence of nucleotides, only one DNA polymerase must be observed in one spot. This is achieved with structures called zero mode waveguides (ZMWs).

The ZMW is a tiny little well, only 100 nm across. The reason they are this small is that only one DNA polymerase will occupy each ZMW. The DNA polymerase is fixed the base of the ZMW with a special layer of biotinylated polyethylene glycol, which stops the DNA polymerase floating away.

Tens of thousands of individual ZMWs are arrayed on a chip. The ZMWs are so small that only very small wavelengths of light are able to enter them, so low wavelength lasers are used to illuminate the array. The resulting fluorescent pulse sequences from the DNA polymerases are recorded by a sophisticated “high-multiplex confocal fluorescence detection system”.

The technology is able to keep track of 3000 ZMWs at once and the DNA polymerases are able to read 5,000 to 8,000 bases in just over an hour. At that rate, you could sequence the entire human genome in a day with just 14,000 ZMWs, though they’d need to upgrade the detection system. Mind you, at $700,000 for Pacific Biosystem’s state of the art RS II SMRT sequencing machine, it’d burn a bit of a hole in your pocket. However, the relentless advance of technology and science will hopefully cut that cost down in the next decade or two.

All in all, SMRT sequencing is an amazing piece of technology, and the whole process is summarised quite nicely in this little video by Pacific Biosystems.

This article was based on Eid et al. (2009) Science. 323:133-138. It is available on the NCBI, here.

Homecoming

I WROTE A DEPRESSING HELIX THING THAT TURNED OUT KINDA LONG

BECAUSE WE NEED MORE DEPRESSING SERGIO THINGS RIGHT NOW, DON’T WE?

Pre-season 2, but with definite (and possible) season 2 spoilers. Ye be warned.

Summary: He survived Arctic BioSystems. He survived the Narvik outbreaks. He got the hell out of Ilaria and didn’t look back.
They didn’t agree with that much.

AO3 Link Here


The moment he opened his eyes - barely squinting, it washard to manage more, they have you drugged - it was easy to figure outthat he was under fluorescent lights again. That was normal enough, thoughSergio wasn’t sure he had been inside before. Things were still fuzzy and it took a few hard blinks to get anything to start clearing up. Really bright lights, grey walls, no furniture other than the chair he was in, one door…he knew these types of rooms.

He just usually wasn’t the one zip-tied in place.

Keep reading

so said, being that economics fails even under the artificial conditions under which it operates, human advancement should be measured not in growth (which is finite), but absolute development, which concerns human wellbeing, and not the construction of properties or products

and no model should use destruction as a mechanism of development

so, really, what i’m saying is, if economics is going to be considered a science, it should be understood and taught as a subset of biosystems engineering

anonymous asked:

what's a good minor for a geological engineering major?

Some of the obvious choices include:

  • Biosystems Engineering
  • Chemistry
  • Engineering
  • Marine Science/Biology
  • Physics
  • Plant Sciences
  • Atmospheric Sciences and Meteorology
  • Environmental Science
  • Geography
  • Landscape Architecture
  • Biology
  • Petroleum Engineering
  • Soil Science

But really anything math or science-y or just whatever you are interested in/passionate about will make a good minor.

So the official name of my college department is “Biosystems and Agricultural Engineering,” which we shorten to BAE, and we call ourselves BAEs (pronouncing the letters in the acronym). 

But this still became downright hilarious when “bae” became slang for “baby” or an adorable person, generally a term of endearment.

We are baes. We are literally all baes.

[Unless you subscribe to one of my friends who swore we should start telling people that the acronym stands for BadA** Engineers. Needless to say, our departmental administration shot that idea down…]

featheredthings

Reuters: Healthcare: BRIEF-Novacyt and Leica Biosystems sign distribution deal for NOVAprep in Greater China

Reuters: Healthcare

Reuters.com is your source for breaking news, business, financial and investing news, including personal finance and stocks. Reuters is the leading global provider of news, financial information and technology solutions to the world’s media, financial institutions, businesses and individuals.

BRIEF-Novacyt and Leica Biosystems sign distribution deal for NOVAprep in Greater China
http://feeds.reuters.com/&
Mar 4th 2015, 06:26

* Announces that Novacyt and Leica Biosystems have signed a distribution deal for NOVAprep in Greater China


You are receiving this email because you subscribed to this feed at https://blogtrottr.com

If you no longer wish to receive these emails, you can unsubscribe here:
https://blogtrottr.com/unsubscribe/gk5/M0SKfw